Molecular cloning, characterization and expression profiles of Annexin family (ANXA1~A6) in yellow catfish (Pelteobagrus fulvidraco) and ANX regulation by CpG ODN responding to bacterial infection
| Autor: | Junhao Jia, Jiatian Wei, Min Zeng, Linghua Zhang, Haiming Cai, Miaopeng Ma, Feiping Ming, Qin Fan, Peijing Zhao, Qianyi Liang, Jinbo Deng, Haokun Shen, Jiayi Li, Zengjue Zhao, Shuxia Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Fish Proteins
0301 basic medicine Annexins CpG Oligodeoxynucleotide Spleen Aquatic Science Biology Microbiology Fish Diseases Interferon-gamma 03 medical and health sciences Ion binding Immune system Immunity medicine Animals Environmental Chemistry Cloning Molecular Catfishes Bacterial Infections 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate Aeromonas hydrophila 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Oligodeoxyribonucleotides CpG site 040102 fisheries 0401 agriculture forestry and fisheries Catfish |
| Zdroj: | Fish & Shellfish Immunology. 99:609-630 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.02.032 |
| Popis: | Up to now, many previous reports have emphasized that Annexins (ANX) family played an important role in immune responses. Aeromonas hydrophila (A. hydrophila), the most common zoonotic pathogenic bacteria of yellow catfish (Pelteobagrus fulvidraco), can cause serious economic loss, especially to yellow catfish with high economic value. In our previous work, we demonstrated that synthetic oligodeoxynucleotides containing CpG motifs (CpG ODN) owned powerful immunostimulatory activity. However, the relationship among Pelteobagrus fulvidraco Annexins (Pf_ANX), CpG ODN and A. hydrophila is unknown. Therefore, we cloned Pf_ANX1-6 genes and analyzed its sequences, structures, genetic evolution, post-translation modifications (PTMs), Ca2+ ion binding sites and tissue distribution to reveal the relevance. In addition, we investigated the responses of ANXA1-6 and cytokines in intestine and spleen as well as morbidity/survival rate of fish post CpG ODN immunization and/or A. hydrophila infection. The results showed that compared with challenge alone (challenge-CK) group, the CpG immunization following challenge (CpG-challenge) group displayed relatively flat IL-1β level throughout in both organs. Meanwhile, the expression of IFN-γ and morbidity/survival rate of fish in CpG-challenge group showed a great improvement compared with the challenge-CK group. Our results indicated that CpG ODN could improve morbidity/survival by up-regulating Pf_ANXA 1, 2 and 5 in the intestine and spleen to ameliorate inflammatory responses and promote anti-infective responses. Our findings offer some important insights into ANX related to the immunity of fish infection and lay a theoretical basis for the prevention and treatment of fish infections. |
| Databáze: | OpenAIRE |
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