Recombinant human follicle-stimulating hormone is capable of exerting a biological effect in the adult hypophysectomized rat by reducing the numbers of degenerating germ cells
| Autor: | Andrzej Bartke, K E Borg, T J Corbin, L R De Franca, P Grasso, Lonnie D. Russell |
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| Rok vydání: | 1993 |
| Předmět: |
Testosterone propionate
Male endocrine system medicine.medical_specialty Hypophysectomy medicine.drug_class medicine.medical_treatment Testicle Biology Follicle-stimulating hormone chemistry.chemical_compound Endocrinology Internal medicine Testis medicine Animals Testosterone Spermatogenesis Sperm Count Leydig Cells Organ Size Seminiferous Tubules Spermatozoa Recombinant Proteins Rats Seminiferous tubule medicine.anatomical_structure chemistry Gonadotropin Follicle Stimulating Hormone |
| Zdroj: | Endocrinology. 133(5) |
| ISSN: | 0013-7227 |
| Popis: | There is considerable controversy as to whether FSH can, under normal circumstances, exert an effect to promote spermatogenesis in the adult rat. Recombinant human FSH (rhFSH) was used to answer a more limited question relating to whether FSH is capable of exerting a biological effect in promoting adult spermatogenesis. Can a pure preparation of FSH prevent the regressive changes seen after hypophysectomy (Hx) in a short term experiment? To answer this question, five groups of adult rats were used as follows: pituitary-intact animals, 3-day hypophysectomized (Hx), 3-day Hx given 3 mg testosterone propionate (T)/day for 7 days, 3-day Hx given 22 IU rhFSH for 7 days, and 3-day Hx given saline vehicle for 7 days. Testis weight, seminiferous tubule diameter, analysis of four degenerating germ cell types, the relative amount of lipid, and the levels of FSH receptors showed that FSH could, in a significant manner, prevent the regressive changes accompanying Hx. FSH was not as effective as T in doing so, because the FSH values were always intermediate between T-maintained animals and those after long term Hx. The Leydig cell was eliminated as a possible source of FSH-stimulated T promotion of spermatogenesis, given that morphometry and tissue T assays indicated that no additional production of T was elicited by rhFSH. The assay system used to enumerate degenerating germ cells proved a very sensitive indicator of the ability of hormones to maintain cell viability in short term experiments. The data not only show that FSH can exert a biological effect, but that this effect is qualitatively similar to that seen after the administration of T in terms of the maintenance of viability of specific germ cell types. A hypothesis is presented whereby FSH and T, although the former acting by a second messenger system and the latter by binding to nuclear receptors, can stimulate the genome to exert similar qualitative effects promoting the viability of germ cells. |
| Databáze: | OpenAIRE |
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