The unsaturation of phosphatidylglycerol in thylakoid membrane alleviates PSII photoinhibition under chilling stress
| Autor: | Qing-Wei Meng, Li-Yan Wang, Qiu-Yu Zhang, Shi-Jie Zhao, Sha Yang, Xiao-lin Sun |
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| Rok vydání: | 2011 |
| Předmět: |
Chlorophyll
Photoinhibition Light Plant Science Biology Thylakoids Superoxide dismutase chemistry.chemical_compound Ascorbate Peroxidases Solanum lycopersicum Oxygen evolving activity chemistry.chemical_classification Phosphatidylglycerol Superoxide Dismutase Fatty Acids Electric Conductivity Photosystem II Protein Complex food and beverages Fatty acid Phosphatidylglycerols Hydrogen Peroxide General Medicine Plants Genetically Modified APX Cold Temperature Chloroplast Biochemistry chemistry Thylakoid Glycerol-3-Phosphate O-Acyltransferase biology.protein Agronomy and Crop Science |
| Zdroj: | Plant Cell Reports. 30:1939-1947 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-011-1102-2 |
| Popis: | Over-expression of chloroplast glycerol-3-phosphate acyltransferase gene (LeGPAT) in tomato increased cis-unsaturated fatty acid content in phosphatidylglycerol (PG) of the thylakoid membrane. Under chilling stress, the oxygen evolving activity, the maximal photochemical efficiency of PSII (F v/F m), and superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities decreased less in sense lines than in antisense lines compared to wild-type (WT) plants. Consistently, the relative electric conductivity, $${\text{O}}_{2} ^{{. - }} $$ and H2O2 contents in sense lines were lower than those of WT and antisense lines. The antisense lines with low level of unsaturated fatty acids in PG were extremely susceptible to photoinhibition of PSII and had a significant reduction in the D1 protein content of PSII reaction center under chilling stress. However, in the presence of streptomycin (SM), the degradation of D1 protein was faster in sense lines than in WT and antisense plants. These results suggested that, under chilling stress conditions, increasing cis-unsaturated fatty acids in PG through over-expression of LeGPAT can alleviate PSII photoinhibition by accelerating the repair of D1 protein and improving the activities of antioxidant enzymes in chloroplasts. |
| Databáze: | OpenAIRE |
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