A novel injectable pH–temperature sensitive hydrogel containing chitosan–insulin electrosprayed nanosphere composite for an insulin delivery system in type I diabetes treatment
Autor: | Dai Phu Huynh, Doo Sung Lee, Thi Cam Thach To, Thai Minh Duy Le, Vu Viet Linh Nguyen, Thuy An Trinh, Hoang Gia Vinh Ho |
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Rok vydání: | 2020 |
Předmět: |
medicine.medical_treatment
Biomedical Engineering macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Oligomer Polyethylene Glycols Chitosan Mice chemistry.chemical_compound Blood plasma medicine Copolymer Animals Insulin General Materials Science Mice Inbred BALB C Lactide Temperature technology industry and agriculture Hydrogels Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Streptozotocin 0104 chemical sciences Diabetes Mellitus Type 1 chemistry 0210 nano-technology Ethylene glycol Nanospheres medicine.drug Nuclear chemistry |
Zdroj: | Biomaterials Science. 8:3830-3843 |
ISSN: | 2047-4849 2047-4830 |
DOI: | 10.1039/d0bm00634c |
Popis: | A novel insulin composite delivery system was prepared and characterized. The composite consisted of a pH- and temperature-sensitive hydrogel, which is an oligomer serine-b-poly(lactide)-b-poly(ethylene glycol)-b-poly(lactide)-b-oligomer serine (OS-PLA-PEG-PLA-OS) pentablock copolymer, as matrix and chitosan-insulin electrosprayed nanospheres (CIN) as constituent materials. The properties of the OS-PLA-PEG-PLA-OS pentablock copolymer and the chitosan-insulin nanoparticles were characterized. The chitosan-insulin nanospheres uniformly distributed in the matrix had a reinforcing effect on the mechanical properties and prolonged the degradation time of the hydrogel depot under body conditions. The composite solutions accommodating different concentrations of the chitosan-insulin nanospheres were subcutaneously injected into induced diabetic BALB/c mice to study the in vivo insulin-release profile. The result showed that insulin concentrations in blood plasma were maintained at a steady-state level. Furthermore, the bio-properties of the insulin were retained and it showed a blood glucose level reducing effect for more than 60 hours after injection to a streptozotocin (STZ)-induced diabetic mouse model. The results suggested that this injectable pH-temperature sensitive hydrogel containing chitosan-insulin electrosprayed nanosphere composites has promising potential applications for type 1 diabetes treatment. |
Databáze: | OpenAIRE |
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