Use of SSR markers to determine the anther-derived homozygous lines in coconut
| Autor: | Deepthi Yakandawala, Jean-Luc Verdeil, Valérie Hocher, L. Perera, L. K. Weerakoon, P. I. P. Perera |
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| Rok vydání: | 2008 |
| Předmět: |
Cocos
Genetic Markers Identification ADN Stamen Plant Science Marker analysis Biology F30 - Génétique et amélioration des plantes Microspore Botany Marqueur génétique Cocos nucifera Dihaploïdie DNA Primers Repetitive Sequences Nucleic Acid Ploidies Base Sequence Homozygote fungi food and beverages Culture d'anthère General Medicine Flow Cytometry Ploidy analysis F02 - Multiplication végétative des plantes Genetic marker Microsatellite Ploidy Agronomy and Crop Science |
| Zdroj: | Plant Cell Reports |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-008-0592-z |
| Popis: | Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs. |
| Databáze: | OpenAIRE |
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