Immunocytochemical localization of Cathepsin D and CA 125 in ovarian cancer
| Autor: | Peter Athanassiades, E. Petrakakou, Stylianos Michalas, Pauline Athanassiadou, V. Sakellariou, D. Aravantinos |
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| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Immunocytochemistry Cathepsin D Ovary Immunoenzyme Techniques Ovarian carcinoma medicine Carcinoma Humans Lymph node Ovarian Neoplasms business.industry Obstetrics and Gynecology General Medicine Prognosis medicine.disease Cystadenocarcinoma Serous Serous fluid medicine.anatomical_structure Receptors Estrogen CA-125 Antigen Lymphatic Metastasis Female Menopause Receptors Progesterone business Ovarian cancer |
| Zdroj: | International Journal of Gynecology & Obstetrics. 56:31-37 |
| ISSN: | 0020-7292 |
| DOI: | 10.1016/s0020-7292(96)02792-0 |
| Popis: | Objective: To study the expression of Cathepsin D (Cath D) and CA 125 antigens and ER and PR receptors on freshly obtained surgical specimens of ovarian carcinomas and their relationship with menopausal status, tumor histology, primary tumor size and lymph node invasion. Method: The tumors obtained from 100 women were measured and cut in half. The cut surface of one half was pressed against glass slides which were air dried and stained using the Avidin-Biotin peroxidase method for Cath D and CA 125 antigens. The slides were viewed under the light microscope for the characteristic brown granules in the cytoplasm or membrane of the malignant cells. The other half of the tumor was subjected to routine histological examination and part used for the demonstration of ER and PR receptors. The results were analyzed using χ2 analysis. Results: Cath D positivity was as common as CA 125 positivity. Cath D positivity is more frequently associated with serous carcinomas than with others. No relationship was observed between ER/PR positivity and Cath D or CA 125 positivity. Conclusion: The high incidence of Cath D positivity makes it a possible complementary method for following up ovarian carcinoma patients especially those who are CA 125 negative. |
| Databáze: | OpenAIRE |
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