Kinetics of bactericidal activity of antibiotics measured by luciferin-luciferase assay
| Autor: | N. Messedi, Y. de Rautlin de la Roy, B. Grignon, G. Grollier |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Staphylococcus aureus
medicine.drug_class Antibiotics Biophysics Microbial metabolism Firefly luciferin Firefly Luciferin Biology medicine.disease_cause chemistry.chemical_compound Adenosine Triphosphate Bacterial Proteins Escherichia coli medicine Extracellular Topoisomerase II Inhibitors Luciferases chemistry.chemical_classification Bacteria Cell Membrane DNA-Directed RNA Polymerases Luciferin Molecular biology Anti-Bacterial Agents Kinetics Enzyme chemistry Biochemistry Chemistry (miscellaneous) Biological Assay Intracellular |
| Zdroj: | Journal of Bioluminescence and Chemiluminescence. 6:193-201 |
| ISSN: | 1099-1271 0884-3996 |
| DOI: | 10.1002/bio.1170060310 |
| Popis: | ATP production, measured by the luciferin-luciferase assay, is an indicator of bacterial metabolic activity. This enzymatic assay yields rapid results (less than 5 minutes), permitting multiple measurements and establishment of ATP growth curves in order to study the kinetics of antibiotics in bacterial populations. The measurement of free or extracellular ATP, total ATP (extra and intracellular) and the ratio of free to total ATP are additional means of studying the bacteriostatic or bactericidal activity of antibiotics. An increase in free ATP is an indicator of extracellular movement due to alteration of the cell wall. The ratio free ATP/total ATP x 100 greater than or equal to 50%, indicates bacteriallysis. These assays were used to study the effects of 14 antibiotics on two reference strains of Escherichia coli ATCC 25922 and Staphylococcus aureus 25923. |
| Databáze: | OpenAIRE |
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