Voltage-dependant anion channels: Novel insights into isoform function through genetic models
| Autor: | Brett H. Graham, Tatiana Sheiko, Adithya Raghavan, William J. Craigen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Gene isoform
Voltage-dependent anion channel Biophysics Mitochondrion Biochemistry Article Genetic model Animals Humans Protein Isoforms Voltage-Dependent Anion Channels Animal model Embryonic Stem Cells Loss function Models Genetic biology Porin Cell Biology Mitochondria Cell biology biology.protein Drosophila Mouse knock out Bacterial outer membrane Function (biology) |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1818:1477-1485 |
| ISSN: | 0005-2736 |
| Popis: | Voltage-dependant Anion Channels, also known as mitochondrial porins, are pore-forming proteins located in the mitochondrial outer membrane (MOM) that, in addition to forming complexes with other proteins that localize to the MOM, also function as the main conduit for transporting metabolites between the cytoplasm and mitochondria. VDACs are encoded by a multi-member gene family, and the number of isoforms and specific functions of VDACs varies between species. Translating the well-described in vitro characteristics of the VDAC isoforms into in vivo functions has been a challenge, with the generation of animal models of VDAC deficiency providing much of the available information about isoform-specific roles in biology. Here, we review the approaches used to create these insect and mammalian animal models, and the conclusions reached by studying the consequences of loss of function mutations on the genetic, physiologic, and biochemical properties of the resulting models. This article is part of a Special Issue entitled: VDAC structure, function, and regulation of mitochondrial metabolism. |
| Databáze: | OpenAIRE |
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