Activation of CD8 T cells by antigen expressed in the pituitary gland
| Autor: | James de Jersey, Iain C. A. F. Robinson, Danielle Carmignac, Brigitta Stockinger, Thomas Barthlott |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cytotoxicity
Immunologic Adoptive cell transfer medicine.medical_specialty Pituitary gland Somatotropic cell T cell Immunology Mice Transgenic Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Autoantigens Mice Anterior pituitary Antigen Cell Movement Pituitary Gland Anterior T-Lymphocyte Subsets Internal medicine MHC class I medicine Immunology and Allergy Cytotoxic T cell Animals Antigens Viral Interphase Antigen Presentation Cell Death Viral Core Proteins RNA-Binding Proteins Dendritic Cells Nucleocapsid Proteins Adoptive Transfer Mice Inbred C57BL medicine.anatomical_structure Endocrinology Nucleoproteins Influenza A virus Organ Specificity Growth Hormone biology.protein Lymph Nodes |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 169(12) |
| ISSN: | 0022-1767 |
| Popis: | Ag expressed exclusively in the anterior pituitary gland and secreted locally by pituitary somatotrophs can gain access to the MHC class I presentation pathway and activate CD8 T cells. Influenza nucleoprotein (NP) was expressed as a transgene under the control of the human growth hormone (GH) locus control region. Activation of monoclonal F5 CD8 T cells specific for NP resulted in spontaneous autoimmune pathology of the pituitary gland in mice transgenic for both NP and the F5 TCR. Destruction of somatotrophs resulted in drastically reduced GH levels in adult mice and a dwarf phenotype. Adoptive transfer of F5 T cells into NP-transgenic hosts resulted in full T cell activation, first demonstrable in regional lymph nodes, followed by their migration to the pituitary gland. Despite the presence of activated, IFN-γ-producing CD8 T cells in the pituitary gland and a slight reduction in pituitary GH levels, no effect on growth was observed. Thus, CD8 T cells have access to the neuroendocrine system and get fully activated in the absence of CD4 help, but Ag recognition in this location causes autoimmune pathology only in the presence of excessive CD8 T cell numbers. |
| Databáze: | OpenAIRE |
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