| Autor: |
Nataliya Petinati, Yulia Davydova, Ksenia Nikiforova, Alexey Bigildeev, Alexander Belyavsky, Georgiy Arapidi, Nina Drize, Larisa Kuzmina, Elena Parovichnikova, Valeriy Savchenko |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Transplantation and Cellular Therapy. 29:109.e1-109.e10 |
| ISSN: |
2666-6367 |
| DOI: |
10.1016/j.jtct.2022.10.030 |
| Popis: |
Multipotent mesenchymal stromal cells (MSCs) are currently under intensive investigation for the treatment and prevention of graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT), owing to their substantial immunomodulatory properties. The responses of recipients to MSC infusion following allo-HSCT are not yet well understood. T cells are central to the adaptive immune system, protecting the organism from infection and malignant cells. Memory T cells with different phenotypes, gene expression profiles, and functional properties are critical for immune processes regulation. The aim of this study was to study the dynamics of memory T cell subpopulations and cytokines in the blood of allo-HSCT recipients after MSC administration. In clinical trial NCT01941394, patients after allo-HSCT were randomized into 2 groups, one receiving standard GVHD prophylaxis and the other also receiving MSC infusion on the day of leukocyte recovery to 1000 cells/μL (engraftment, day E0). Blood samples of patients from both groups were analyzed on days E0, E+3, and E+30. T cell subpopulations were studied by flow cytometry, and cytokine concentrations were evaluated by the Bio-Plex Pro Human Cytokine Panel. Administration of MSCs to patients on day E0 did not affect the overall dynamics of restoration of absolute numbers and proportions of T and B lymphocytes after 3 and 30 days. At 3 days after MSC injection, only the numbers of CD8 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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