Treatment with a neutralizing anti-murine interleukin-17 antibody after the onset of coxsackievirus b3-induced viral myocarditis reduces myocardium inflammation
| Autor: | Wu Weifeng, Yang Fan, Pang Yu, Kong Qing, Yan Yuluan, Huang Yanlan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Myocarditis T cell Inflammation Biology Antibodies Viral Proinflammatory cytokine lcsh:Infectious and parasitic diseases Mice Virology medicine Animals lcsh:RC109-216 Mice Inbred BALB C Research Myocardium Interleukin-17 Interleukin Antibodies Monoclonal medicine.disease Antibodies Neutralizing Enterovirus B Human Infectious Diseases medicine.anatomical_structure Immunology biology.protein Tumor necrosis factor alpha Interleukin 17 medicine.symptom Antibody |
| Zdroj: | Virology Journal Virology Journal, Vol 8, Iss 1, p 17 (2011) |
| ISSN: | 1743-422X |
| Popis: | Background Recently, some studies indicate that interleukin (IL)-17, known as a T cell (Th17)-derived proinflammatory cytokine, is the major mediator of tissue inflammation in inflammatory and autoimmune diseases. Viral myocarditis (VMC) is a T cell-mediated autoimmune disease, but the role for IL-17 in VMC is not well defined. Results Using IL-17 monoclonal antibody (IL-17mAb)-treated VMC mice, we tested the pathogenic role of IL-17 in the development of VMC. VMC mice were treated with monoclonal rat anti-murine IL-17 antibody (anti-IL-17) or rat IgG2A isotype control or phosphate-buffered solution 3 days after Coxsackievirus B3 (CVB3) injection. Normal mice without any manipulation were taken as normal control. The survival rates of mice were monitored and heart pathology was examined histologically. IL-17, IL-6, and TNF-α mRNA of the myocardium were assessed by semi-quantitative RT-PCR. Systemic IL-17, IL-6, and TNF-α level were measured by enzyme-linked immunosorbent assay, and local myocardium IL-17 expression was analyzed using immunohistochemical staining. Flow cytometric analysis was used to evaluate the frequencies of Th17 subsets in CD4+T cells. Results showed that neutralization of IL-17 with anti-IL-17 can ameliorate clinical symptoms, defer disease course, decrease serum IL-17 level, without declining the IL-17, IL-6 and TNF-α mRNA transcript level and serum IL-6, TNF-α level. The differentiation and proliferation of the Th17 cells were unchanged. Conclusions Our data suggest that IL-17 is crucially involved in the pathogenesis of murine VMC, IL-17 inhibition might ameliorate the myocardium inflammation after the onset of VMC. |
| Databáze: | OpenAIRE |
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