Identification of a consensus DNA-binding site for the TCP domain transcription factor TCP2 and its important roles in the growth and development of Arabidopsis
| Autor: | Jiamin Chen, Jing Xiang, Suchun Liu, Zihao Zeng, Xiaomei Zhou, Lingting Yin, Zhimin He |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Time Factors DNA Plant Arabidopsis Cyclopentanes Flowers medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Protein Domains Gene Expression Regulation Plant Consensus Sequence Morphogenesis Genetics medicine Oxylipins Binding site Molecular Biology Gene Transcription factor Mutation Binding Sites Base Sequence biology Arabidopsis Proteins General Medicine Circadian Clock Associated 1 biology.organism_classification Hypocotyl Cell biology Plant Leaves DNA binding site 030104 developmental biology 030220 oncology & carcinogenesis Leaf morphogenesis Protein Binding Signal Transduction Transcription Factors |
| Zdroj: | Molecular Biology Reports. 48:2223-2233 |
| ISSN: | 1573-4978 0301-4851 |
| Popis: | TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR 1 (TCP) transcription factors control multiple aspects of growth and development in various plant species. However, few genes were reported to be directly targeted and regulated by them through their specific binding sites, and then uncover their functions in plants. A consensus DNA-binding site motif of TCP2 was identified by random binding site selection (RBSS). DNA recognized by TCP2 contained the motif G(G/T)GGNCC(A/C), which showed high consistency with motifs bound by other TCP domain proteins. Consequently, this motif was regarded as the specific DNA-binding sites of TCP2. Circadian clock associated 1 (CCA1) and EARLY FLOWERING 3 (ELF3) were subsequently considered as potential target genes owing to the containing of the similar TCP2 binding sites or core binding sites GGNCC and found to be positively regulated by TCP2 via DNA binding. Phenotype analysis results showed that mutation and over-expression of TCP2 resulted in variations in leaf morphogenesis, especially the double or triple mutations of TCP2, 4 and 10. Mutations in TCPs caused late flowering. Finally, TCP2 was shown to influence hypocotyl elongation by mediating the jasmonate signaling pathway. Overall, these results provide a basis for future studies aimed at distinguishing the target genes of TCP2 and elucidating the important roles of TCP2 in plant growth and development. |
| Databáze: | OpenAIRE |
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