Altered cannabinoid receptor mRNA expression in peripheral blood mononuclear cells from marijuana smokers
| Autor: | Thomas W. Klein, Herman Friedman, Liang Nong, Qingwen Cheng, Michael D. Roth, Catherine Newton |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Male medicine.medical_specialty Cannabinoid receptor Receptors Drug medicine.medical_treatment Immunology Down-Regulation Gene Expression Marijuana Smoking Biology Peripheral blood mononuclear cell Basal (phylogenetics) Sex Factors Internal medicine Ethnicity medicine Humans Immunology and Allergy RNA Messenger Receptors Cannabinoid Receptor Gene Messenger RNA Age Factors Middle Aged In vitro Endocrinology Neurology Leukocytes Mononuclear Female lipids (amino acids peptides and proteins) Neurology (clinical) Cannabinoid Mitogens |
| Zdroj: | Journal of Neuroimmunology. 127:169-176 |
| ISSN: | 0165-5728 |
| Popis: | We studied, using RT-PCR, the relative expression of cannabinoid receptor (CBR) mRNA in peripheral blood mononuclear cells (PBMC) from different donor groups. Cells from normal donors expressed a CB2 mRNA level threefold higher than CB1 across all age, gender or ethnicity groups, and amplicons were of the same size in all donors. However, cells from marijuana users expressed higher levels of CBR mRNA, but with a preserved CB1/CB2 ratio of 1:3. CBR gene products were also studied following short-term mitogen activation in vitro. CB1 expression decreased following mitogen stimulation when compared to the time-matched medium only cells while the expression of CB2 mRNA remained unchanged. These studies suggest that marijuana smoking and immune activation can alter the basal levels of CB1 and CB2 in PBMCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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