Influence of altered O2 tension on substrate metabolism in perfused rat lung
| Autor: | Eskew Ml, Rhoades Ra, M E Shaw |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
medicine.medical_specialty Glucose uptake Palmitates Palmitic Acids Biology chemistry.chemical_compound Simulated altitude Physiology (medical) Internal medicine medicine Animals Hypoxia Lung Glycogen Fasting Metabolism respiratory system Environment Controlled Lipid Metabolism In vitro Rats Oxygen Perfusion Increased lactate Glucose Endocrinology medicine.anatomical_structure chemistry Lactates |
| Zdroj: | American Journal of Physiology-Legacy Content. 229:1476-1479 |
| ISSN: | 0002-9513 |
| DOI: | 10.1152/ajplegacy.1975.229.6.1476 |
| Popis: | Effects of hypoxia (1.5 h) on glucose and palmitate metabolism were investigated in perfused lungs from normal rats and rats exposed for 24 h to hypobaric conditions (simulated altitude of 24,000 ft). Hypoxic lungs were ventilated with 5% O2-5% CO2 and control lungs with 21% O2-5% CO2. Blood gases and pH remained stable during the 1.5-h perfusion period. Exposure of normal rat lungs to 1.5 h of in vitro hypoxia (blood Po2=34 mmHg) significantly increased lactate production and mean arterial pulmonary pressure, but did not alter glucose uptake, pyruvate levels, and oxidation of either [U-14C]glucose or [1-14C]palmitate to CO2. Incorporation of labeled glucose and palmitate into lung lipids was also unaltered. In contrast to normal lungs, prior exposure to hypoxia for 24 h and subsequent perfusion under hypoxic conditions significantly stimulated glucose uptake (74% increase), markedly increased glucose incorporation into lung lipids, and increased oxidation of glucose to CO2. Lactate/pyruvate ratios also showed a significant 38% increase. Lung glycogen was unchanged following 24 h hypoxia. These data indicate that adaptive changes occur in metabolic processes within the lung during acute changes in O2 tension. |
| Databáze: | OpenAIRE |
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