XB130 enhances invasion and migration of human colorectal cancer cells by promoting epithelial‑mesenchymal transition
| Autor: | Jiancheng Shen, Yonglin Liu, Chang’e Jin, Jie Li, Jinrong Liu, Heping Rao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Epithelial-Mesenchymal Transition Cell Vimentin Biochemistry 03 medical and health sciences 0302 clinical medicine Cell Movement Internal medicine Cell Line Tumor Genetics medicine Humans Epithelial–mesenchymal transition RNA Small Interfering Molecular Biology Protein kinase B XB130 Adaptor Proteins Signal Transducing Cell Proliferation Oncogene biology Cell cycle 030104 developmental biology medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Gene Knockdown Techniques biology.protein Cancer research Molecular Medicine Colorectal Neoplasms Signal Transduction |
| Zdroj: | Molecular medicine reports. 16(4) |
| ISSN: | 1791-3004 |
| Popis: | The expression of XB130 is associated with invasion and migration of many tumor cells, but its roles in human colorectal cancer (CRC) remains unknown. To investigate this, protein expression levels of XB130 in numerous human CRC cell lines were compared with a normal colorectal mucosa cell line by western blotting. Knockdown of XB130 using small interfering (si)RNA was performed to assess the effects on cell invasion and migration in a Transwell assay and a scratch test. Western blotting was also used to quantify the levels of proteins associated with epithelial‑mesenchymal transition (EMT), including E‑cadherin, vimentin, phosphorylated (p)‑protein kinase B (AKT), p‑forkhead homeobox type O 3a (FOXO3a) and zinc finger E‑box‑binding homeobox 1 (ZEB‑1). The relative expression of XB130 protein was significantly higher in CRC cells compared with control cells (P |
| Databáze: | OpenAIRE |
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