Functional Integrity and Gene Expression Profiles of Human Cord Blood-Derived Hematopoietic Stem and Progenitor Cells Generated In Vitro
| Autor: | Roberto Dircio-Maldonado, Odette Balvanera-Ortiz, Ileana Mondragón-García, Alberto Cedro-Tanda, Julieta Corral-Navarro, Hector Mayani, Fredy Omar Beltrán-Anaya, Lourdes Arriaga-Pizano, Patricia Flores-Guzmán, Alfredo Hidalgo-Miranda |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Myeloid Cord Blood CD34 Cell Culture Techniques Ex vivo expansion Hematopoietic stem and progenitor cells Antigens CD34 Biology CD38 03 medical and health sciences 0302 clinical medicine Translational Research Articles and Reviews Antigens CD Receptors Transferrin medicine Humans Progenitor cell Cells Cultured Oligonucleotide Array Sequence Analysis Stem Cells Cell Biology General Medicine Fetal Blood Hematopoietic Stem Cells Cell biology Transplantation Haematopoiesis 030104 developmental biology medicine.anatomical_structure Gene expression profiles Hematopoietic Stem/Progenitor Cells 030220 oncology & carcinogenesis Cord blood Leukocyte Common Antigens Stem cell Transcriptome Hematopoietic Regeneration Cell Culture Advances Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine |
| ISSN: | 2157-6564 |
| Popis: | To date, different experimental strategies have been developed for the ex vivo expansion of human hematopoietic stem (HSCs) and progenitor (HPCs) cells. This has resulted in significant advances on the use of such expanded cells in transplantation settings. To this day, however, it is still unclear to what extent those stem and progenitor cells generated in vitro retain the functional and genomic integrity of their freshly isolated counterparts. In trying to contribute to the solving of this issue, in the present study we have selected and purified three different hematopoietic cell populations: HSCs (CD34+ CD38− CD45RA− CD71− Lin− cells), myeloid progenitor cells (CD34+ CD38+ CD45RA+ CD71− Lin− cells), and erythroid progenitor cells (CD34+ CD38+ CD45RA− CD71+ Lin− cells), obtained directly from fresh human umbilical cord blood (UCB) units or generated in vitro under particular culture conditions. We, then, compared their functional integrity in vitro and their gene expression profiles. Our results indicate that in spite of being immunophenotipically similar, fresh and in vitro generated cells showed significant differences, both in functional and genetic terms. As compared to their fresh counterparts, those HSCs generated in our culture system showed a deficient content of long-term culture-initiating cells, and a marked differentiation bias toward the myeloid lineage. In addition, in vitro generated HSCs and HPCs showed a limited expansion potential. Such functional alterations correlated with differences in their gene expression profiles. These observations are relevant in terms of HSC biology and may have implications in UCB expansion and transplantation. |
| Databáze: | OpenAIRE |
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