RNA-seq based gene expression analysis of ovarian granulosa cells exposed to zearalenone in vitro: significance to steroidogenesis
| Autor: | Yu-Feng Wang, Chuan-Liang Ji, Guo-Liang Zhang, Yan-Zhong Feng, Wei Ge, Shun-Feng Cheng, Wei Shen, Jie Yu, Shi-Duo Sun, Xiao-Feng Sun, Yong Zhao, Lan Li, Rui-Qian Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system steroidogenesis medicine.medical_treatment IER3 RNA-Seq Biology Steroid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Gene expression medicine Gene Zearalenone food and beverages porcine Molecular biology In vitro 030104 developmental biology Endocrinology Oncology chemistry granulosa cells 030220 oncology & carcinogenesis gene expression RNA-seq Hormone Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Zearalenone (ZEA) is a natural contaminant of various food and feed products representing a significant problem worldwide. Since the occurrence of ZEA in grains and feeds is frequent, the present study was carried out to evaluate the possible effects of ZEA on steroid production and gene expression of porcine granulosa cells, using RNA-seq analysis. Porcine granulosa cells were administered 10 μM and 30 μM ZEA during 72 h of culture in vitro. Following ZEA treatment the gene expression profile of control and exposed granulosa cells was compared using RNA-seq analysis. The results showed that in the exposed granulosa cells ZEA significantly altered the transcript levels, particularly steroidogenesis associated genes. Compared with the control group, 10 μM and 30 μM ZEA treatment significantly increased the mRNA expression of EDN1, IER3, TGFβ and BDNF genes and significantly reduced the mRNA expression of IGF-1 and SFRP2 genes. In particular, ZEA significantly decreased the expression of genes essential for estrogen synthesis including FSHR, CYP19A1 and HSD17β in granulosa cells. Furthermore, Q-PCR and Western-blot analysis also confirmed reduced expression of these genes in ZEA exposed granulosa cells. These effects were associated with a significant reduction of 17β-estradiol concentrations in the culture medium of granulosa cells. Collectively, these results demonstrated a concretely deleterious effect of ZEA exposure on the mRNA expression of steroidogenesis related genes and the production of steroid hormones in porcine ovarian granulosa cells in vitro. |
| Databáze: | OpenAIRE |
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