Nondestructive Chemical Analysis of the Iron-Containing Protein Ferritin Using Raman Microspectroscopy
| Autor: | Natalia P. Ivleva, Martin Elsner, Carolin Hartmann, Reinhard Niessner |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Maghemite
02 engineering and technology engineering.material Spectrum Analysis Raman 010402 general chemistry Ferric Compounds 01 natural sciences Ferrihydrite chemistry.chemical_compound Mössbauer spectroscopy Animals Horses Magnetite Nanoparticles Instrumentation Spectroscopy Magnetite biology 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Ferritin chemistry Ferritins X-ray crystallography biology.protein engineering 0210 nano-technology Bacteria Intracellular Nuclear chemistry |
| Zdroj: | Applied Spectroscopy. 74:193-203 |
| ISSN: | 1943-3530 0003-7028 |
| DOI: | 10.1177/0003702818823203 |
| Popis: | Ferritin is a ubiquitous intracellular iron storage protein of animals, plants, and bacteria. The cavity of this protein acts like a reaction chamber for natural formation and storage of nano-sized particles via biomineralization. Knowledge of the chemical composition and structure of the iron core is highly warranted in the fields of nano technologies as well as biomolecules and medicine. Here, we show that Raman microspectroscopy (RM) is a suitable nondestructive approach for an analysis of proteins containing such nano-sized iron oxides. Our approach addresses: (1) synthesis of suitable reference materials, i.e., ferrihydrite, maghemite and magnetite nanoparticles; (2) optimization of parameters for Raman spectroscopic analysis; (3) comparison of Raman spectra from ferritin with apoferritin and our reference minerals; and (4) validation of Raman analysis by X-ray diffraction and Mössbauer spectroscopy as two independent complementary approaches. Our results reveal that the iron core of natural ferritin is composed of the iron(III) hydroxide ferrihydrite (Fe2O3 ∙ 0.5 H2O). |
| Databáze: | OpenAIRE |
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