Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection
| Autor: | Nicola Luchi, Aida Raio, Alberto Santini, Chiara Aglietti, Paolo Capretti, Alessia Lucia Pepori, Paola Bartolini, Francesco Pecori |
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| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
Pathogen detection lcsh:Biotechnology lcsh:QR1-502 Biophysics Loop-mediated isothermal amplification Portable diagnostics Olive Quick Decline Syndrome 01 natural sciences Applied Microbiology and Biotechnology Alien pathogens Canker Stain Disease Isothermal amplification LAMP Sudden Oak Death lcsh:Microbiology law.invention 03 medical and health sciences Phytophthora ramorum law lcsh:TP248.13-248.65 010608 biotechnology Quarantine medicine Natural ecosystem 030304 developmental biology 0303 health sciences biology Warning system Ceratocystis platani biology.organism_classification Virology medicine.drug_formulation_ingredient Original Article Xylella fastidiosa Isothermal amplifcation |
| Zdroj: | AMB Express AMB express 9 (2019). doi:10.1186/s13568-019-0774-9 info:cnr-pdr/source/autori:Aglietti C., Luchi N., Pepori A.L., Bartolini P., Pecori F., Raio A., Capretti P., Santini A./titolo:Real-time loop-mediated isothermal amplifcation: an early-warning tool for quarantine plant pathogen detection/doi:10.1186%2Fs13568-019-0774-9/rivista:AMB express/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:9 AMB Express, Vol 9, Iss 1, Pp 1-14 (2019) |
| ISSN: | 2191-0855 |
| DOI: | 10.1186/s13568-019-0774-9 |
| Popis: | An effective framework for early warning and rapid response is a crucial element to prevent or mitigate the impact of biological invasions of plant pathogens, especially at ports of entry. Molecular detection of pathogens by using PCR-based methods usually requires a well-equipped laboratory. Rapid detection tools that can be applied as point-of-care diagnostics are highly desirable, especially to intercept quarantine plant pathogens such as Xylella fastidiosa, Ceratocystis platani and Phytophthora ramorum, three of the most devastating pathogens of trees and ornamental plants in Europe and North America. To this aim, in this study we developed three different loop mediated isothermal amplification (LAMP) assays able to detect each target pathogen both in DNA extracted from axenic culture and in infected plant tissues. By using the portable instrument Genie® II, the LAMP assay was able to recognize X. fastidiosa, C. platani and P. ramorum DNA within 30 min of isothermal amplification reaction, with high levels of specificity and sensitivity (up to 0.02 pg µL−1 of DNA). These new LAMP-based tools, allowing an on-site rapid detection of pathogens, are especially suited for being used at ports of entry, but they can be also profitably used to monitor and prevent the possible spread of invasive pathogens in natural ecosystems. |
| Databáze: | OpenAIRE |
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