Residual radiation effect in the murine hematopoietic stem cell compartment
| Autor: | H.-P. Peterson, K. H. von Wangenheim, L. E. Feinendegen |
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| Rok vydání: | 1986 |
| Předmět: |
Male
Cell division Cell Biophysics Spleen Mice Inbred Strains Biology Andrology Colony-Forming Units Assay Mice Idoxuridine medicine Doubling time Animals General Environmental Science Radiation Cell growth Hematopoietic stem cell Hematopoietic Stem Cells Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Gamma Rays Immunology Female Stem cell Cell Division |
| Zdroj: | Radiation and environmental biophysics. 25(2) |
| ISSN: | 0301-634X |
| Popis: | Stem cells surviving radiation injury may carry defects which contribute to long-term effects. The ratio of 125-iododeoxyuridine (IUdR) uptake into spleens of lethally irradiated recipient mice between day 3 and day 5 after cell transfusion revealed reduced proliferative ability (PF) of spleen seeding cells in parallel with reduced CFU-S content of donors throughout the study period of one year after 5 Gy gamma irradiation. Additional data aided in evaluating possible mechanisms of PF reduction. Within the range of the graft sizes used, PF was independent of the numbers of cells or CFU-S transfused. Radiation-induced increase in loss of label between days 3 and 5 and prolonged doubling time of proliferating cells indicated enhancement of cell maturation and increase in mitotic cycle time. Increased IUdR uptake per transfused CFU-S suggested extra divisions of transit cells due to insufficiency in the stem cell compartment. It is concluded that persisting defects in surviving stem cells interfere in a complex way with cell proliferation in the hemopoietic system. |
| Databáze: | OpenAIRE |
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