Replication of Hepatitis B Virus Which Carries Foreign DNA in Vitro
| Autor: | Toshiyuki Shinji, Norio Koide, Tadashi Hanafusa, Takao Tsuji, Yasuhiro Yumoto, Hajime Hada |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
DNA Replication
Hepatitis B virus HBV RNA encapsidation signal epsilon Genes Viral Hepatitis B virus DNA polymerase Genetic Vectors Green Fluorescent Proteins Molecular Sequence Data Biophysics Biology Transfection medicine.disease_cause Biochemistry Hepatitis B virus PRE beta Virus Tumor Cells Cultured medicine Humans Viral Regulatory and Accessory Proteins Vector (molecular biology) Molecular Biology Base Sequence DNA replication virus diseases DNA virus Cell Biology Virology Molecular biology digestive system diseases Luminescent Proteins Mutagenesis Insertional Liver DNA Viral Gene Targeting Trans-Activators Gene Deletion Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 262:530-533 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1999.1224 |
| Popis: | Targeting a specific DNA sequence to the desired tissues is an important step in gene therapy. The hepatitis B virus (HBV) is the only DNA virus that has hepatocyte specificity. We attempted to construct an HBV-based vector for targeting the liver. We observed the replication and secretion of virus particles in an HBV construct that lacks X gene and carries an extra 63 bp DNA fragment in vitro. Replication was observed in the cell line HuH-7 but not HepG2. From this construct, we designed an HBV-based vector that could carry foreign DNA. HBV based vectors provide for the possibilities of generating therapeutic agents for individual patients. Our host vector system may be used to clear out the HBV from the HBV carrier or chronic hepatitis B patients by introducing a genetically engineered HBV into these patients. |
| Databáze: | OpenAIRE |
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