Glucose as a Protein-Condensing Cellular Solute

Autor:	Naotaka Noda, Yejin Jung, Genyir Ado, Yoshiyuki Mizuhata, Masakazu Higuchi, Tetsuya Ogawa, Fumiyoshi Ishidate, Shin-ichi Sato, Hiroki Kurata, Norihiro Tokitoh, Motonari Uesugi
Rok vydání:	2022
Předmět:	Proteomics Glucose Calmodulin Glycogenolysis Homeostasis Molecular Medicine General Medicine Biochemistry
Zdroj:	ACS Chemical Biology. 17:567-575
ISSN:	1554-8937 1554-8929
DOI:	10.1021/acschembio.1c00849
Popis:	The present study reports a surprising protein-condensing effect of glucose, prompted by our accidental observation during chemical library screening under a high-glucose condition. We noticed "glucosing-out" of certain compounds, in which physiological concentrations of glucose induced compound aggregation. Adapting the "glucosing-out" concept to proteins, our proteomic analysis identified three cellular proteins (calmodulin, rho guanine nucleotide exchange factor 40, and polyubiquitin-C) that displayed robust glucose-dependent precipitation. One of these proteins, calmodulin, formed glucose-dependent condensates that control cellular glycogenolysis in hepatic cells. Our findings suggest that glucose is a heretofore underappreciated driver of protein phase separation that may have profound effects on cellular homeostasis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a93bf91a37baaf86999b340e470d869 https://doi.org/10.1021/acschembio.1c00849 Zobrazit plný text záznamu