Detection of Platelet-Monocyte Aggregates by the ADAM® Image Cytometer
| Autor: | Jeong Ah Yoon, Bo Kyeung Jung, Kyung Chul Moon, Chi Hyun Cho, Soo Young Yoon, Dae Sung Hur |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Blood Platelets Male Pathology medicine.medical_specialty Adolescent Platelet Aggregation Monocytes Flow cytometry Young Adult medicine Humans Platelet Platelet activation Child Aged Cell Aggregation Fluorescent Dyes Whole blood Alternative methods Reproducibility Short Research Communication medicine.diagnostic_test business.industry Monocyte Reproducibility of Results Thrombosis General Medicine Middle Aged Flow Cytometry Platelet Activation Peripheral blood carbohydrates (lipids) medicine.anatomical_structure ADAM® Child Preschool Female platelet-monocyte aggregates image cytometer business Algorithms Biomedical engineering |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Background: Inappropriate platelet activation is known to be associated with various thrombotic disorders. Platelet-monocyte aggregates (PMAs), whose formation is mediated by platelet surface P-selectin (CD62P), can be used as a reliable marker to detect platelet activation. Previous studies have generally detected PMAs through flow cytometry-based approaches. Recently, the ADAM ® image cytometer (Nanoentek Inc., Seoul, Korea) was developed for image-based cellular analysis. In this study, we detected PMAs with the ADAM ® cytometer, evaluated the reproducibility of the measurements made by the ADAM ® cytometer, and compared the abilities of the ADAM ® cytometer and a flow cytometric assay to detect PMAs. Methods: Whole blood samples were collected from patients. Within 5 minutes of collection, anticoagulated whole blood samples were fixed in 10% paraformaldehyde and 5% glyoxal. Nineteen clinical specimens were collected; each was analyzed three times with the ADAM ® cytometer in order to assess the reproducibility of its measurements. To compare the ability of the ADAM ® cytometer with that of a flow cytometer to detect PMAs, each cytometer was used for 23 clinical samples and the correlation of the measurements was determined. Results: The PMA measurements made by the ADAM ® cytometer showed good reproducibility (CV < 10% for all specimens). Moreover, the PMA measurements made by the ADAM ® cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944). Conclusions: The ADAM ® cytometer is a suitable alternative method to the flow cytometry-based assays. Since the ADAM cytometer does not need specialized instrument knowledge or software proficiency (unlike flow cytometry), the ADAM ® cytometer can be used as a rapid and reliable POCT device to measure platelet activation in peripheral blood. This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases. ® , platelet-monocyte aggregates, platelet activation |
| Databáze: | OpenAIRE |
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