Gas chromatography–mass spectrometry/mass spectrometry analysis to determine natural and post-administration levels of oestrogens in bovine serum and urine
| Autor: | P. Teale, J. Bowman, S. Biddle, A. Robinson, Edward Houghton |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Quality Control Analyte Ether Urine Mass spectrometry Sensitivity and Specificity Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Anabolic Agents Sex Factors Animals Environmental Chemistry Bovine serum albumin Spectroscopy Chemical ionization Chromatography biology Reproducibility of Results Estrogens Models Chemical chemistry Calibration biology.protein Cattle Female Gas chromatography Gas chromatography–mass spectrometry |
| Zdroj: | Analytica Chimica Acta. 586:115-121 |
| ISSN: | 0003-2670 |
| Popis: | A novel analytical approach has been developed and shown to be capable of detecting the isomers of oestradiol in the low ppt (pg mL(-1)) range in bovine serum and urine. Following extractive derivatisation the analytes were detected as their 3-pentafluorobenzoyl 17-trimethylsilyl ether derivatives by gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS), using electron capture negative ion chemical ionisation. The isomers of oestradiol were quantified in both blank and post-administration urine and serum samples, with a view to setting action/threshold levels for these compounds, to allow discrimination between normal samples and samples from animals treated with growth promoting ear implants. A non-parametric statistical assessment of the data resulted in proposed action levels (with a false positive probability of 1 in 1000) of 1.6 and 2.7 ng mL(-1) for 17alpha-oestradiol, in male and female urine, respectively, and 40 and 44 pg mL(-1) for 17beta-oestradiol, in male and female urine, respectively. An action level of 20 pg mL(-1) was proposed for 17alpha- and 17beta-oestradiol in male serum. In female serum the proposed action levels were 40 and 20 pg mL(-1) for 17alpha- and 17beta-oestradiol, respectively. |
| Databáze: | OpenAIRE |
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