Maternal Nicotine Exposure Exacerbates Neonatal Hyperoxia-Induced Lung Fibrosis in Rats
| Autor: | Tsu Fu Yeh, Chung Ming Chen, Chun Mao Lin, Liang Ti Huang, Hsiu Chu Chou |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Nicotine
medicine.medical_specialty Pathology Time Factors Pulmonary Fibrosis medicine.medical_treatment Connective tissue Gestational Age Hyperoxia Rats Sprague-Dawley Pregnancy Risk Factors Internal medicine medicine Animals Nicotinic Agonists Lung Fetus integumentary system business.industry Growth factor Connective Tissue Growth Factor respiratory system medicine.disease respiratory tract diseases CTGF Disease Models Animal Endocrinology medicine.anatomical_structure Animals Newborn Maternal Exposure Prenatal Exposure Delayed Effects Pediatrics Perinatology and Child Health Female Collagen medicine.symptom business Developmental Biology medicine.drug |
| Zdroj: | Neonatology. 106:94-101 |
| ISSN: | 1661-7819 1661-7800 |
| Popis: | Background: Maternal nicotine exposure increases lung collagen in fetal and newborn animals. Connective tissue growth factor (CTGF) plays a role in hyperoxia-induced pulmonary fibrosis. Objective: To determine whether pre- and postnatal nicotine exposure can augment CTGF expression and postnatal hyperoxia-induced lung fibrosis. Methods: Nicotine was administered to pregnant Sprague-Dawley rats at a dose of 6 mg/kg/day from gestational days 7-21 (prenatal nicotine-treated group) and gestational day 7 to postnatal day 14 (pre- and postnatal nicotine-treated group). A control group of pregnant dams was injected with an equal volume of saline. Within 12 h of birth, rats were exposed to room air or 1 week of >95% O2 and an additional 2 weeks of 60% O2 (3 weeks of hyperoxia). Lungs were taken for total collagen, CTGF expression and histological analyses. Results: In each maternal treatment group, the rats reared in hyperoxia had a higher total collagen compared with rats reared in room air on postnatal days 7 and 21. Collagen content was significantly higher in rats born to pre- and postnatal nicotine-treated dams than rats born to saline-treated and prenatal nicotine-treated dams on postnatal days 7 and 21. Pre- and postnatal nicotine exposure and neonatal hyperoxia exposure increased CTGF expression on postnatal days 7 and 21. Conclusions: CTGF may be involved in the pathogenesis of lung fibrosis induced by maternal nicotine and neonatal hyperoxia, and maternal nicotine exposure exacerbates neonatal hyperoxia-induced lung fibrosis. These results are relevant to neonates who require supplemental oxygen and are exposed to the breast milk of smoking mothers during infancy. |
| Databáze: | OpenAIRE |
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