Purification of chitosanases produced by Bacillus toyonensis CCT 7899 and functional oligosaccharides production
| Autor: | Cristiane Fernandes de Assis, José A. Teixeira, Nayara Sousa da Silva, Julia Maria de Medeiros Dantas, Everaldo Silvino dos Santos, Leandro de Santis Ferreira, Allanny Alves Furtado, Manoela Torres-Rêgo, Nathália Kelly de Araújo, Renata Mendonça Araújo, Matheus F. Fernandes-Pedrosa |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
medicine.drug_class
Chitosanases Intragastric route 02 engineering and technology Biochemistry Anti-inflammatory Chitooligosaccharides Chitosan 03 medical and health sciences chemistry.chemical_compound Hydrolysis medicine 030304 developmental biology chemistry.chemical_classification 0303 health sciences Science & Technology General Medicine 021001 nanoscience & nanotechnology 6. Clean water 3. Good health Enzyme chemistry 0210 nano-technology Bacillus toyonensis Biotechnology Paw edema |
| Popis: | Chitooligosaccharides (COS) have a great potential to be used by pharmaceutical industry due to their many biological activities. The use of enzymes to produce them is very advantageous, however it still faces many challenges, such as discovering new strains capable to produce enzymes that are able to generate bioactive oligosaccharides. In the present study a purification protein protocol was performed to purify chitosanases produced by Bacillus toyonensis CCT 7899 for further chitosan hydrolysis. The produced chitooligosaccharides were characterized by mass spectroscopy (MS) and their antiedematogenic effect was investigated through carrageenan-induced paw edema model. The animals were treated previously to inflammation by intragastric route with COS at 30, 300 and 600mg/kg. The purification protocol showed a good performance for the chitosanases purification using 0.20M NaCl solution to elute it, with a 9.54-fold purification factor. The treatment with COS promoted a decrease of paw edema at all evaluated times and the AUC04h, proving that COS produced showed activity in acute inflammation like commercial anti-inflammatory Dexamethasone (corticosteroid). Therefore, the strategy used to purification was successfully applied and it was possible to generate bioactive oligosaccharides with potential pharmacological use. The authors thank CAPES and Brazilian National Council for Research [Grant 401817/2016-4] for the financial supporting. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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