Microfluidic devices manufacturing with a stereolithographic printer for biological applications
| Autor: | C. Bao-Varela, Maria Teresa Flores-Arias, A. I. Gómez-Varela, Ezequiel Álvarez, Bastian Carnero |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, Universidade de Santiago de Compostela. Departamento de Física Aplicada |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fabrication
Materials science Stereolithography Biocompatibility Microfluidics Pillar 3D printing Low force stereolithography Bioengineering Nanotechnology Soft lithography law.invention Biomaterials chemistry.chemical_compound Organ-on-a-chip HUVEC law PDMS Lab-On-A-Chip Devices Human Umbilical Vein Endothelial Cells Humans Microchannel Polydimethylsiloxane business.industry chemistry Mechanics of Materials Printing Three-Dimensional business |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| Popis: | Stereolithographic printers have revolutionized many manufacturing processes with their capacity to easily produce highly detailed structures. In the field of microfluidics, this technique avoids the use of complex steps and equipment of the conventional technologies. The potential of low force stereolithography technology is analysed for the first time using a Form 3B printer and seven printing resins through the fabrication of microchannels and pillars. Manufacturing performance of internal and superficial channels and pillars is studied for the seven printing resins in different configurations. A complete characterization of printed structures is carried out by optical, confocal and SEM microscopy, and EDX analysis. Internal channels with unobstructed lumen are obtained for diameters and angles greater than 500 μm and 60°, respectively. Outward and inward superficial channels in the range of hundreds of microns can be fabricated with an accurate profile, printing them with a perpendicular orientation respect to the base, allowing a proper uncured resin evacuation. Outward channels are replicated by soft lithography using polydimethylsiloxane. Clear, Model and Tough resins show a good behaviour to be used as master, but Amber and Dental resins present a poor topology transference from the master to the replica. According to the needs of devices used for biological and biomedical research, transparency as well as superficial biocompatibility of some resins is evaluated. Human umbilical vein endothelial cells (HUVEC) adhesion is confirmed on Amber, Dental and Clear resins, but these cells were only able to grow and progress as a cell culture over the Amber resin. Therefore, Amber showed an adequate biocompatibility, in terms of cell adhesion and growth for HUVEC Authors gratefully thank contracts AEI RTI2018-097063-B-100, AEI/FEDER, UE; ED431B 2020/29; ED431E 2018/08 and ED481D-2021-019, Consellería de Educación Xunta de Galicia/FEDER e Estructuración Xunta de Galicia, IN607A2019-02 and Sociedad española de cardiología y Fundación español del corazón, SEC/FEC-INV-BAS 20/013 SI |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|