EFFECTS OF BOVINE POLYMERIZED HEMOGLOBIN ON COAGULATION IN CONTROLLED HEMORRHAGIC SHOCK IN SWINE
Autor: | Nora Philbin, Ludmila Asher, Daniel Freilich, Bruce L. Pearce, William S. Flournoy, Feng Dong, Carol Nicholson, Richard M. McCarron, Mike Hammett, Franc xoise Arnaud, Jennifer Rice |
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Rok vydání: | 2005 |
Předmět: |
Blood Platelets
Resuscitation Bleeding Time Time Factors Blood transfusion Polymers Swine medicine.medical_treatment Hemorrhage Shock Hemorrhagic Sodium Chloride Critical Care and Intensive Care Medicine Blood substitute Hydroxyethyl Starch Derivatives Hemoglobins Necrosis Blood serum Intensive care Animals Medicine Blood Coagulation Lung Fibrin Hemostasis medicine.diagnostic_test business.industry Myocardium Hydrogen-Ion Concentration Thromboelastography Thrombelastography Oxygen Microscopy Electron Hematocrit Anesthesia Lactates Prothrombin Time Emergency Medicine Acidosis Lactic Cattle Partial Thromboplastin Time business Partial thromboplastin time |
Zdroj: | Shock. 24:145-152 |
ISSN: | 1073-2322 |
DOI: | 10.1097/01.shk.0000170354.18437.2f |
Popis: | HBOC-201, a bovine polymerized hemoglobin, has been proposed as a novel oxygen-carrying resuscitative fluid for patients with hemorrhagic shock (HS). Herein, we evaluated the hemostatic effects of HBOC-201 in an animal model of HS. A 40% blood loss-controlled hemorrhage and soft tissue injury were performed in 24 invasively monitored Yucatan mini-pigs. Pigs were resuscitated with HBOC-201 (HBOC) or hydroxyethyl starch (HEX), or were not resuscitated (NON) based on cardiac parameters during a 4-h prehospital phase. Afterward, animals received simulated hospital care for 3 days with blood or saline transfusions. Hemostasis measurements included in vivo bleeding time (BT), thromboelastography (TEG), in vitro bleeding time (platelet function; PFA-CT), prothrombin time (PT), and partial thromboplastin time (PTT). Serum lactate was measured and lung sections were evaluated for microthrombi by electron microscopy. During the prehospital phase, BT remained unchanged in the HBOC group. TEG reaction time increased in HBOC pigs during the late prehospital phase and was greater than in NON or HEX pigs at 24 h (P = 0.03). TEG maximum amplitude was similar for the two fluid-resuscitated groups. PFA-CT increased in both resuscitated groups but less with HBOC (P = 0.02) in the prehospital phase; this effect was reversed by 24 h (P = 0.02). In the hospital phase, PT decreased (P < 0.02), whereas PTT increased above baseline (P < 0.01). Lactic acidosis in HBOC and HEX groups was similar. Aspartate aminotransferase was relatively elevated in the HBOC group at 24 h. Electron microscopy showed no evidence of platelet/fibrin clots or microthrombi in any of the animals. Twenty-four-hour group differences mainly reflected the fact that all HEX animals (8/8) received blood transfusions compared with only one HBOC animal (1/8). In swine with HS, HBOC resuscitation induced less thrombopathy than HEX during the prehospital phase. Mild delayed effects on platelet and clot formation during the hospital phase are transient and likely related to fewer blood transfusions. In swine with HS, HBOC resuscitation induced less thrombopathy than HEX during the prehospital phase but more thrombopathy in the hospital phase. The delayed effects on platelet and clot formation during the hospital phase are transient and may be related to the need for fewer blood transfusions. KEYWORDS—Blood substitutes, hemoglobin-based oxygen carriers, trauma, coagulopathy, resuscitation |
Databáze: | OpenAIRE |
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