Loss of chromosome arms 3p and 9p and inactivation of P16 (INK4a) in normal epithelium of patients with primary lung cancer
| Autor: | James G. Herman, Qing Liu, Daniel M. Cohen, Otavia L. Caballero, James M. Engles, Jin Jen, Manel Esteller, Christoph Lengauer, Julie Bonacum, Robert Yochem, David Sidransky, William H. Westra, Peter White |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Lung Neoplasms Bronchi Respiratory Mucosa Biology Biopsy Genetics medicine Humans Gene Silencing Lung cancer Cyclin-Dependent Kinase Inhibitor p16 medicine.diagnostic_test Genes p16 Chromosome Cancer medicine.disease Epithelium medicine.anatomical_structure Immunology Respiratory epithelium Immunohistochemistry Female Chromosomes Human Pair 3 Chromosome Deletion Chromosomes Human Pair 9 Fluorescence in situ hybridization |
| Zdroj: | Genes, chromosomescancer. 32(2) |
| ISSN: | 1045-2257 |
| Popis: | The accumulation of genetic alterations in the respiratory epithelium may give rise to cancer and often is accompanied by a series of histologic alterations over a period of several years. Recent studies have identified some molecular alterations in histologically normal-appearing epithelium among patients with lung cancer. To extend these observations, we investigated clonal genetic alterations by using fluorescence in situ hybridization (FISH) analysis and immunohistochemistry in 69 biopsy samples of histologically normal-appearing bronchial epithelium from 22 patients with or without lung cancer. Thirty-seven biopsy specimens from 13 patients were examined for loss of 3p14, and 48 biopsy specimens from 18 patients were examined for loss at 9p21 by FISH. P16INK4a expression was analyzed in 54 biopsy samples from 19 patients. In at least one biopsy specimen from five of the13 patients with primary lung cancer, FISH or immunohistochemistry detected loss of the 3p14 or 9p21 region. In contrast, no alterations were detected for the same regions in the nine patients without primary lung cancer. Our results support the concept that the normal epithelial surface of large bronchi of patients with lung cancer has molecular changes suggestive of the outgrowth of numerous clonal foci. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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