α-Lipoic acid ameliorates oral mucositis and oxidative stress induced by methotrexate in rats. Histological and immunohistochemical study

Autor: Norhan M. El-Sayed, Amal A.M. Ahmed, Manar A.A. Selim
Rok vydání: 2016
Předmět:
musculoskeletal diseases
0301 basic medicine
Diarrhea
Male
Pathology
medicine.medical_specialty
medicine.medical_treatment
Intraperitoneal injection
Pharmacology
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Mucositis
Medicine
Animals
General Pharmacology
Toxicology and Pharmaceutics
Rats
Wistar
skin and connective tissue diseases
Lamina propria
Stomatitis
biology
Thioctic Acid
business.industry
Mouth Mucosa
General Medicine
Buccal administration
DNA
medicine.disease
Immunohistochemistry
Proliferating cell nuclear antigen
Rats
Lipoic acid
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Methotrexate
chemistry
030220 oncology & carcinogenesis
Toxicity
biology.protein
business
Oxidative stress
Biomarkers
Zdroj: Life sciences. 171
ISSN: 1879-0631
Popis: Aim Oral mucositis is a common adverse effect of Methotrexate (MTX) that may limit its clinical use. Oxidative stress and apoptosis have been proposed to mediate MTX toxicity. The current study was conducted to assess the conceivable protective effect of α-lipoic acid (LA) against MTX induced toxicity on both buccal and lingual mucosae. Main methods Thirty male Wistar rats were allocated into three groups; control, MTX-treated group subjected to single intraperitoneal injection of MTX (20 mg/kg, i.p.) and LA- treated group treated with daily intraperitoneal injection of LA (10 mg/kg, i.p.) for 5 weeks before MTX injection (20 mg/kg, i.p.). Rats were then sacrificed under anesthesia then their buccal and lingual mucosae were dissected out and processed for biochemical and histopathological studies. Biomarkers of oxidative stress and integrity of nuclear DNA (nDNA) were estimated. Immunostaining was used to determine Bax and PCNA localization. Key findings MTX-treated rats showed increased levels of MDA and fragmentation of DNA in addition to reduction of GSH levels and activities of catalase and SOD. Histological examination of MTX-treated rats demonstrated degenerative changes that involved the surface epithelium and lamina propria of their buccal and lingual mucosae. Immunohistochemical results of MTX-treated rats revealed strongly positive Bax and weakly positive PCNA staining reactivity of the nuclei of the basal and parabasal cells of the surface epithelium. However, LA significantly attenuated MTX-evoked alterations in the previous-stated parameters highlighting its antioxidant and anti-apoptotic potential. Significance LA may be suggested to be a prospective candidate to ameliorate MTX-induced oral mucositis.
Databáze: OpenAIRE
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