Filter paper disk techniques for assay of nucleotidase
| Autor: | Heinz C. Schröder, Klaus Dose, Rudolf K. Zahn, Josef Arendes, Wernere G. Müller |
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| Rok vydání: | 1977 |
| Předmět: |
Paper
Nucleotidase activity Thymus Gland Substrate Specificity Nucleotidases Column chromatography Nucleotidase Methods Genetics Animals Nucleotide Molecular Biology chemistry.chemical_classification Chromatography Filter paper Nucleotides Nucleosides General Medicine Adenosine Monophosphate Kinetics chemistry Biochemistry Filter (video) Cattle Nucleoside |
| Zdroj: | Molecular Biology Reports. 3:331-337 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/bf00420391 |
| Popis: | A DE filter disk technique for assaying the activity of nucleotidase is described. This method is based on the observation that nucleotides bind to the filters at 5 mM Tris-HCl (pH 7.8) while nucleosides do not. As parameter for the nucleotidase activity the decrease of bound nucleotides is determined. In parallel experiments the amount of the product (nucleoside) formed can be measured by DEAE Sephadex column chromatography. The filter disk technique can be applied for the determination of vmax and Km of a nucleotidase by using different ribonucleosidase monophosphate substrates. |
| Databáze: | OpenAIRE |
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