High mobility group proteins HMG1 and HMG2 do not decrease the melting temperature of DNA
| Autor: | Lyuben N. Marekov, Ladislav Pivec, Beltcho G. Beltchev |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Chemistry
Chromosomal Proteins Non-Histone Melting temperature Biophysics Analytical chemistry High Mobility Group Proteins Cell Biology DNA Nucleic Acid Denaturation Biochemistry Free dna Light scattering chemistry.chemical_compound High-mobility group Animals Thermodynamics Denaturation (biochemistry) Cattle Molecular Biology |
| Zdroj: | Biochemical and biophysical research communications. 120(3) |
| ISSN: | 0006-291X |
| Popis: | High mobility group proteins 1 and 2 isolated in non-denaturing conditions cannot decrease the temperature of denaturation of DNA. When they are isolated or treated with tricloroacetic acid a hyperchromic peak below the melting temperature of free DNA appears in agreement with previous data ( Javaherian et al. (1979) Nucl . Acids Res. 6, 3569-3580). We show that this is due to light scattering of aggregated protein at submelting temperatures and not to melting of DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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