The Role of CTGF in Inflammatory Responses Induced by Silica Particles in Human Bronchial Epithelial Cells

Autor: Hui Lin, Qimei Yu, Yuewei Liu, Guoqing Fu, Ling Zhang, Yuqin Shi, Lingzhi Qin, Ting Zhou, Lu Lei, Zhenyu Wang
Rok vydání: 2019
Předmět:
Pulmonary and Respiratory Medicine
medicine.medical_treatment
Blotting
Western
Interleukin-1beta
Connective tissue
Inflammation
Bronchi
Enzyme-Linked Immunosorbent Assay
Respiratory Mucosa
Real-Time Polymerase Chain Reaction
Proinflammatory cytokine
Transforming Growth Factor beta1
03 medical and health sciences
0302 clinical medicine
Fibrosis
medicine
Humans
030212 general & internal medicine
RNA
Messenger
integumentary system
Chemistry
Interleukin-6
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Growth factor
Interleukin-17
Interleukin-8
Connective Tissue Growth Factor
Epithelial Cells
Transfection
medicine.disease
Silicon Dioxide
Molecular biology
CTGF
medicine.anatomical_structure
030228 respiratory system
Gene Knockdown Techniques
medicine.symptom
Intracellular
Zdroj: Lung. 197(6)
ISSN: 1432-1750
Popis: Prolonged exposure to crystalline silica leads to persistent pulmonary inflammation and progressive fibrosis. Connective tissue growth factor (CTGF) has emerged as a potent proinflammatory and profibrotic regulator to participate in a variety of chronic inflammatory diseases. However, the role of CTGF in silica-induced pulmonary inflammation remains poorly understood. To explore the effect of CTGF on inflammatory responses caused by silica particles, human bronchial epithelial cells (16HBE) were transfected with CTGF siRNA and exposed to silica particles at concentrations of 0, 12.5, 25, 50, 100 μg/ml for 48 h. Intracellular CTGF mRNA and protein expressions were determined by RT-PCR and Western blotting, respectively. The levels of inflammatory cytokines including IL-8, TNF-α, IL-6, IL-1β, IL-17A and TGF-β1 were measured by ELISA kits. Silica particles induce significantly elevated intracellular CTGF mRNA expression in 16HBE cells in a dose-dependent manner when compared with blank control group (P
Databáze: OpenAIRE
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