Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid
| Autor: | Arnold Geis, Jan Kok, B. J. Hayema, M J van Belkum, Gerhardus Venema |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
DNA
Bacterial Genetic Vectors Restriction Mapping Streptococcaceae Molecular cloning Applied Microbiology and Biotechnology Plasmid Restriction map Shuttle vector Bacteriocin Bacteriocins Cloning Molecular Ecology biology Lactococcus lactis subsp cremoris Lactococcus lactis Nucleic Acid Hybridization biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Restriction enzyme Blotting Southern bacteria DNA Probes Food Science Biotechnology Research Article Plasmids |
| Zdroj: | Applied and environmental microbiology, 55(5), 1187-1191. AMER SOC MICROBIOLOGY |
| ISSN: | 0099-2240 |
| Popis: | Lactococcus lactis subsp. cremoris 9B4 plasmid p9B4-6 (60 kilobases [kb]), which specifies bacteriocin production and immunity, was analyzed with restriction endonucleases, and fragments of this plasmid were cloned into shuttle vectors based on the broad-host-range plasmid pWVO1. Two regions on p9B4-6 were identified which specify inhibitory activity on L. lactis indicator strains: one that could be confined to a 1.8-kb ScaI-ClaI fragment with low antagonistic activity and a 15-kb XbaI-SalI fragment specifying high antagonistic activity. The inhibitory substances produced by these two clones were sensitive to proteolysis. A 4-kb HindIII fragment derived from the 15-kb fragment strongly hybridized with the 1.8-kb fragment. The antagonistic activity specified by the 4-kb fragment was somewhat reduced as compared with that of the 15-kb fragment. A 1.3-kb ScaI-HindIII subfragment of the 4-kb fragment contained both the immunity and bacteriocin genes. Inhibition studies showed that the two bacteriocins had different specificities. |
| Databáze: | OpenAIRE |
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