The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani
| Autor: | Sakari Kauppinen, Anders H. Pederson, Elizabeth Golightly, Palle Schneider, Stephen H. Brown, Kim M. Brown, Torben Halkier, Jill Angela Wahleithner, Feng Xu |
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| Rok vydání: | 1996 |
| Předmět: |
Transcription
Genetic Copper protein Molecular Sequence Data Genes Plant Polymerase Chain Reaction law.invention Rhizoctonia Rhizoctonia solani law Complementary DNA Genetics Amino Acid Sequence Gene Laccase chemistry.chemical_classification biology Intron Gene Amplification Sodium Dodecyl Sulfate General Medicine biology.organism_classification Molecular biology Recombinant Proteins Isoenzymes Blotting Southern Enzyme chemistry Recombinant DNA Electrophoresis Polyacrylamide Gel Oxidoreductases |
| Zdroj: | Current genetics. 29(4) |
| ISSN: | 0172-8083 |
| Popis: | Four distinct laccase genes, lcc1, lcc2, lcc3 and lcc4, have been identified in the fungus Rhizoctonia solani. Both cDNA and genomic copies of these genes were isolated and characterized. Hybridization analyses indicate that each of the four laccase genes is present in a single copy in the genome. The R. solani laccases can be divided into two groups based on their protein size, intron/exon organization, and transcriptional regulation. Three of these enzymes have been expressed in the fungus Aspergillus oryzae. Two of the recombinant laccases, r-lcc1 and r-lcc4, as well as the native lcc4 enzyme were purified and characterized. The purified proteins are homodimeric, comprised of two subunits of approximately 66kDa for lcc4 and 50-100kDa for the recombinant lcc1 protein. These laccases have spectral properties that are consistent with other blue copper proteins. With syringaldazine as a substrate, lcc4 has optimal activity at pH7, whereas lcc1 has optimal activity at pH6. |
| Databáze: | OpenAIRE |
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