Labeling of mesenchymal stromal cells with iron oxide–poly(l-lactide) nanoparticles for magnetic resonance imaging: uptake, persistence, effects on cellular function and magnetic resonance imaging properties

Autor: Markus Rojewski, Cédric Ménard, Volker Rasche, Markus Urban, Natalie Fekete, Hubert Schrezenmeier, Gerlinde Schmidtke-Schrezenmeier, Erika Deak, Anna Musyanovych, Karin Tarte, Volker Mailänder, Katharina Landfester
Rok vydání: 2011
Předmět:
Cancer Research
Pathology
medicine.medical_specialty
Injections
Subcutaneous
Immunology
Endosomes
Biology
Mesenchymal Stem Cell Transplantation
Endocytosis
Ferric Compounds
Regenerative medicine
Dioxanes
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
medicine
Animals
Humans
Immunology and Allergy
Rats
Wistar
Genetics (clinical)
030304 developmental biology
0303 health sciences
Transplantation
Staining and Labeling
medicine.diagnostic_test
Mesenchymal stem cell
Mesenchymal Stem Cells
Magnetic resonance imaging
Cell Biology
Magnetic Resonance Imaging
Rats
3. Good health
Radiography
Oncology
chemistry
030220 oncology & carcinogenesis
Biophysics
Feasibility Studies
Nanoparticles
Agarose
Original Article
Intracellular
Stem Cell Transplantation
Homing (hematopoietic)
Zdroj: Cytotherapy; Vol 13
Cytotherapy
ISSN: 1465-3249
DOI: 10.3109/14653249.2011.571246
Popis: Background aims. Mesenchymal stromal cells (MSC) are the focus of research in regenerative medicine aiming at the regulatory approval of these cells for specific indications. To cope with the regulatory requirements for somatic cell therapy, novel approaches that do not interfere with the natural behavior of the cells are necessary. In this context in vivo magnetic resonance imaging (MRI) of labeled MSC could be an appropriate tool. Cell labeling for MRI with a variety of different iron oxide preparations is frequently published. However, most publications lack a comprehensive assessment of the noninterference of the contrast agent with the functionality of the labeled MSC, which is a prerequisite for the validity of cell-tracking via MRI. Methods.We studied the effects of iron oxide-poly(L-lactide) nanoparticles in MSC with flow cytom-etry, transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), Prussian blue staining, CyQuant® proliferation testing, colony-forming unit-fibroblast (CFU-F) assays, flow chamber adhesion testing, immuno-logic tests and differentiation tests. Furthermore iron-labeled MSC were studied by MRI in agarose phantoms and Wistar rats. Results. It could be demonstrated that MSC show rapid uptake of nanoparticles and long-lasting intracellular persistence in the endosomal compartment. Labeling of the MSC with these particles has no influence on viability, differentiation, clonogenicity, proliferation, adhesion, phenotype and immunosuppressive properties. They show excellent MRI properties in agarose phantoms and after subcutaneous implantation in rats over several weeks. Conclusions. These particles qualify for studying MSC homing and trafficking via MRI.
Databáze: OpenAIRE
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