Chondroprotective effects of a new glucosamine combination in rats: Gene expression, biochemical and histopathological evaluation
| Autor: | Nizamettin Koçkara, Nezahat Kurt, Hasan Dogan, Cihat Dundar, Cengiz Ozturk, Halis Suleyman, Aynur Arslan, Murat Türkoğlu, Nihal Cetin, Ebru Sener, Nuray Celik, Yilmaz Ucuncu |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Methylsulfonylmethane Antioxidant Knee Joint medicine.medical_treatment Interleukin-1beta Osteoarthritis Pharmacology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Glucosamine Formaldehyde Gene expression medicine Animals Harpagophytum Dimethyl Sulfoxide Chondroitin sulfate Sulfones General Pharmacology Toxicology and Pharmaceutics Rats Wistar Plant Extracts Tumor Necrosis Factor-alpha Cartilage Chondroitin Sulfates General Medicine medicine.disease Bromelains Rats Disease Models Animal Drug Combinations medicine.anatomical_structure chemistry Biochemistry Gene Expression Regulation |
| Zdroj: | Life sciences. 130 |
| ISSN: | 1879-0631 |
| Popis: | Aims This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates this combination in comparison with another combination of glucosamine hydrochloride, chondroitin sulfate and methylsulfonylmethane (GKM). Materials and methods Animals in the control group were injected with formalin into the knee joint (FCG). Animals in the GCMHB-500 group were given 500 mg/kg GCMHB + formalin, and those in the GKM-500 group were given 500 mg/kg GKM + formalin. Finally, a healthy group (HG) was also used. GCMHB and GKM were administered to rats orally once a day for 30 days. At the end of this period, the rats were sacrificed and the levels of MDA, NO, 8-OH/Gua, and tGSH in the knee joint tissue were measured. Analysis of IL-1β and TNF-α gene expression was done and the tissue was evaluated histopathologically. Key findings MDA, NO and 8-OH/Gua levels and IL-1β and TNF-α gene expression were significantly lower in the GCMHB-500 group compared to the FCG group, whereas tGSH was significantly higher in the GCMHB-500 group than in the FCG group. No significant difference was found for the IL-1β, TNF-α and oxidant/antioxidant parameters between the GKM and FCG groups. The histopathological analysis showed that GCMHB could prevent damage to the cartilage joint, whereas GKM could not. Significance GCMHB may be used clinically by comparing with GKM in the treatment of osteoarthritis. |
| Databáze: | OpenAIRE |
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