Charge detection mass spectrometry on human-amplified fibrils from different synucleinopathies
| Autor: | Aikaterini Tsirkou, Flora Kaczorowski, Mathieu Verdurand, Rana Raffoul, Jonathan Pansieri, Isabelle Quadrio, Fabien Chauveau, Rodolphe Antoine |
|---|---|
| Přispěvatelé: | Spectrométrie des biomolécules et agrégats (SPECTROBIO), Institut Lumière Matière [Villeurbanne] (ILM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Radiopharmaceutical and Neurochemical Biomarkers (BIORAN), Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Nuffield Department of Clinical Neurosciences [Oxford], University of Oxford, VIDAL, Armelle |
| Rok vydání: | 2022 |
| Předmět: |
Amyloid
[CHIM.ANAL] Chemical Sciences/Analytical chemistry Synucleinopathies Metals and Alloys Brain Parkinson Disease General Chemistry Mass Spectrometry Catalysis Surfaces Coatings and Films Electronic Optical and Magnetic Materials Protein Aggregates [CHIM.ANAL]Chemical Sciences/Analytical chemistry alpha-Synuclein Materials Chemistry Ceramics and Composites Humans [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] |
| Zdroj: | Chemical Communications Chemical Communications, 2022, 58 (51), pp.7192-7195. ⟨10.1039/D2CC00200K⟩ |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | International audience; Amyloid fibrils are self-assembled mesoscopic protein aggregates, which can accumulate to form deposits or plaques in the brain. In vitro amplification of fibrils can be achieved with real-time quaking-induced conversion (RT-QuIC). However, this emerging technique would benefit from a complementary method to assess structural properties of the amplification products. This work demonstrates the feasibility of nanospray-charge-detection-mass-spectrometry (CDMS) performed on α-synuclein (αSyn) fibrils amplified from human brains with Parkinson's disease (PD) or Dementia with Lewy bodies (DLB) and its synergistic combination with RT-QuIC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|