Stimulating role of toxoids-laden liposomes in oral immunization against diphtheria and tetanus infections
| Autor: | H. Mirchamsy, Zohreh Hamzeloo, Mohammad Hamedi, P. Ahourai, Hamid Manhouri, G. Fateh |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male food.ingredient Diphtheria Toxoid medicine.medical_treatment Drug Compounding Injections Subcutaneous Immunization Secondary Administration Oral Bioengineering Aluminum Hydroxide complex mixtures Applied Microbiology and Biotechnology Lecithin Microbiology chemistry.chemical_compound food Adjuvants Immunologic Chlorocebus aethiops medicine Tetanus Toxoid Animals Humans Aged Pharmacology Diphtheria toxin Liposome General Immunology and Microbiology Tetanus Aluminium hydroxide Diphtheria Vaccination Toxoid General Medicine Middle Aged medicine.disease chemistry Liposomes Female Rabbits Adjuvant Biotechnology |
| Zdroj: | Biologicals : journal of the International Association of Biological Standardization. 24(4) |
| ISSN: | 1045-1056 |
| Popis: | Liposomes have been produced by injecting an ether solution of a mixture of lecithin and cholesterol into a diluted solution of prewarmed diphtheria and tetanus toxoids followed by elimination of the stream of ether vapour by vacuum. In a preliminary study, adjuvant effects of liposomes on the systemic and mucosal immune response have been studied. When a mixture of diphtheria toxoid (DT) and tetanus toxoid (TT) entrapped in liposomes were administered parenterally or orally in rabbit, a significant rise of specific antibodies against both toxoids was noticed. In monkeys receiving a mixture of DT and TT entrapped in liposomes orally, the antibody response after two and three ingestions of this product was mild but when liposomes containing toxoids were adsorbed with aluminium hydroxide in a similar experiment, a significant rise in the specific antibody response in monkey against both toxoids was recorded. Adult volunteers, similarly receiving a mixture of DT and TT, entrapped in liposomes and adsorbed with aluminium hydroxide have shown a significant rise in specific circulating antitoxins. In order to compare the efficacy of this technique of human oral immunization with the previous method, whereby a plant medicinal seed (LRS) was used as adjuvant in oral immunization of man, a second group of volunteers were simultaneously and similarly treated as suggested previously. The comparative results are discussed in the present report. |
| Databáze: | OpenAIRE |
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