Inward rectification by hyperpolarization-activated Na current in the marine ciliate Euplotes vannus
| Autor: | Thomas Krüppel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Time Factors
Physiology Potassium Sodium Biophysics Euplotes chemistry.chemical_element Mineralogy Lithium Sodium Channels Membrane Potentials chemistry.chemical_compound Animals Egtazic Acid Ciliary membrane Cells Cultured Membrane potential Ciliate Dose-Response Relationship Drug Ethanol biology Tetraethylammonium Depolarization Cell Biology Tetraethylammonium Compounds Hyperpolarization (biology) biology.organism_classification EGTA chemistry Calcium |
| Zdroj: | The Journal of Membrane Biology. 133 |
| ISSN: | 1432-1424 0022-2631 |
| DOI: | 10.1007/bf00232025 |
| Popis: | The ionic mechanisms underlying inward or anomalous rectification have been studied in the marine hypotrichous ciliate Euplotes vannus. Inward-current pulses of moderate amplitude elicited time-dependent rectification that started from a hyperpolarization peak and was expressed as a depolarizing sag towards rest. Voltage-clamp analysis showed that this depolarization is caused by the activation of a complex inward current that does not inactivate with time. The current is carried by a major Na and a minor K component. The Na-current component has been identified by its concentration-dependent reduction in low extra-cellular Na solutions and the capability of Li2+ as Na substitute to carry the current, though with a slightly reduced amplitude. The K-current component has been isolated from the total current after the replacement of Na2+ within the experimental solution. It was blocked in media that contained 10 mmol/liter TEA, a well-known blocker for K inwardly rectifying currents. TEA was only effective at membrane potentials close to or negative to the potassium equilibrium potential. The inward current was reduced after the injection of the Ca chelator EGTA into the cell. Also the elimination of the ciliary membrane, by deciliating cells with ethanol, reduced the amplitude of the inwardly rectifying currents. Both experiments indicate a regulatory function of Ca22+ in inward rectification. |
| Databáze: | OpenAIRE |
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