Graphene-on-Glass Preparation and Cleaning Methods Characterized by Single-Molecule DNA Origami Fluorescent Probes and Raman Spectroscopy
| Autor: | Izabela Kaminska, Philip Tinnefeld, Evelyn Ploetz, Florian Selbach, Patrick Schüler, Johann Bohlen, Renukka Yaadav, Stefan Krause, Florian Steiner |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science General Physics and Astronomy Nanotechnology 02 engineering and technology 010402 general chemistry Spectrum Analysis Raman 01 natural sciences law.invention symbols.namesake law Microscopy DNA origami General Materials Science Fluorescent Dyes Quenching (fluorescence) Graphene General Engineering DNA 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Characterization (materials science) symbols Graphite 0210 nano-technology Raman spectroscopy |
| Zdroj: | ACS nano. 15(4) |
| ISSN: | 1936-086X |
| Popis: | Graphene exhibits outstanding fluorescence quenching properties that can become useful for biophysics and biosensing applications, but it remains challenging to harness these advantages due to the complex transfer procedure of chemical vapor deposition-grown graphene to glass coverslips and the low yield of usable samples. Here, we screen 10 graphene-on-glass preparation methods and present an optimized protocol. To obtain the required quality for single-molecule and super-resolution imaging on graphene, we introduce a graphene screening method that avoids consuming the investigated sample. We apply DNA origami nanostructures to place fluorescent probes at a defined distance on top of graphene-on-glass coverslips. Subsequent fluorescence lifetime imaging directly reports on the graphene quality, as deviations from the expected fluorescence lifetime indicate imperfections. We compare the DNA origami probes with conventional techniques for graphene characterization, including light microscopy, atomic force microscopy, and Raman spectroscopy. For the latter, we observe a discrepancy between the graphene quality implied by Raman spectra in comparison to the quality probed by fluorescence lifetime quenching measured at the same position. We attribute this discrepancy to the difference in the effective area that is probed by Raman spectroscopy and fluorescence quenching. Moreover, we demonstrate the applicability of already screened and positively evaluated graphene for studying single-molecule conformational dynamics on a second DNA origami structure. Our results constitute the basis for graphene-based biophysics and super-resolution microscopy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|