Age-Related Reference Intervals for Blood Amino Acids in Thai Pediatric Population Measured by Liquid Chromatography Tandem Mass Spectrometry
| Autor: | Kanya Suphapeetiporn, Vorasuk Shotelersuk, Santi Punnahitananda, Sirinuch Chomtho, Jaraspong Uaariyapanichkul, Pannee Chinjarernpan, Orapa Suteerojntrakool |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Arginine Article Subject Endocrinology Diabetes and Metabolism Population Physiology Phenylalanine 01 natural sciences High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound Medicine education lcsh:RC620-627 chemistry.chemical_classification education.field_of_study Nutrition and Dietetics Methionine business.industry 010401 analytical chemistry 0104 chemical sciences Amino acid lcsh:Nutritional diseases. Deficiency diseases 030104 developmental biology chemistry Isoleucine Leucine business Research Article Food Science |
| Zdroj: | Journal of Nutrition and Metabolism Journal of Nutrition and Metabolism, Vol 2018 (2018) |
| ISSN: | 2090-0724 |
| DOI: | 10.1155/2018/5124035 |
| Popis: | Background. Age, race, and analytic method influence levels of blood amino acids, of which reference intervals are required for the diagnosis and management of inherited metabolic disorders.Objectives. To establish age-specific reference intervals for blood amino acids in Thai pediatric population measured by liquid chromatography tandem mass spectrometry (LC-MS/MS).Methods. A cross-sectional study of 277 healthy children from birth to 12 years was conducted. Anthropometric, clinical, and dietary information were recorded. Dried blood spots on a filtered paper were used for measurement by derivatized LC-MS/MS. Factors that might affect amino acids such as fasting time and dietary intake were analyzed using quantile regression analysis.Results. Levels of thirteen blood amino acids were reported as median and interval from 2.5th–97.5th percentiles. Compared with those of Caucasian, most blood amino acid levels of Thai children were higher. Compared with a previous study using HPLC in Thai children, many amino acid levels are different. Glycine, alanine, leucine/isoleucine, and glutamic acid sharply decreased after birth. Citrulline, arginine, and methionine stayed low from birth throughout childhood, whereas phenylalanine was at middle level and slightly increased during preadolescence.Conclusion. Reference intervals of age-specific blood amino acids using LC-MS/MS were established in the Thai pediatric population. They diverge from previous studies, substantiating the recommendation that, for the optimal clinical practice, age-specific reference intervals of amino acids should be designated for the particular population and analysis method. |
| Databáze: | OpenAIRE |
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