Transcriptome Sequencing in the Preoptic Region of Rat Dams Reveals a Role of Androgen Receptor in the Control of Maternal Behavior
Autor: | Éva Renner, Vivien Csikós, Fanni Dóra, Edina Brigitta Udvari, Dávid Keller, Árpád Dobolyi, András H. Lékó, Rashmi Kumari |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Steroid hormone receptor Mothers RNA-Seq Biology Catalysis Article Inorganic Chemistry lcsh:Chemistry rat dams 03 medical and health sciences 0302 clinical medicine Internal medicine Gene expression medicine Animals Physical and Theoretical Chemistry hypothalamus Maternal Behavior Molecular Biology lcsh:QH301-705.5 Spectroscopy Messenger RNA Sequence Analysis RNA Gene Expression Profiling Organic Chemistry Postpartum Period Antagonist General Medicine steroid receptor Preoptic Area Computer Science Applications Rats Preoptic area Androgen receptor 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation Hypothalamus Receptors Androgen gene expression Female postpartum mother 030217 neurology & neurosurgery |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 1517, p 1517 (2021) |
ISSN: | 1422-0067 |
Popis: | (1) Background: Preoptic region of hypothalamus is responsible to control maternal behavior, which was hypothesized to be associated with gene expressional changes. (2) Methods: Transcriptome sequencing was first applied in the preoptic region of rat dams in comparison to a control group of mothers whose pups were taken away immediately after parturition and did not exhibit caring behavior 10 days later. (3) Results: Differentially expressed genes were found and validated by quantitative RT-PCR, among them NACHT and WD repeat domain containing 1 (Nwd1) is known to control androgen receptor (AR) protein levels. The distribution of Nwd1 mRNA and AR was similar in the preoptic area. Therefore, we focused on this steroid hormone receptor and found its reduced protein level in rat dams. To establish the function of AR in maternal behavior, its antagonist was administered intracerebroventricularly into mother rats and increased pup-directed behavior of the animals. (4) Conclusions: AR levels are suppressed in the preoptic area of mothers possibly mediated by altered Nwd1 expression in order to allow sustained high-level care for the pups. Thus, our study first implicated the AR in the control of maternal behaviors. |
Databáze: | OpenAIRE |
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