Transcriptome Sequencing in the Preoptic Region of Rat Dams Reveals a Role of Androgen Receptor in the Control of Maternal Behavior

Autor: Éva Renner, Vivien Csikós, Fanni Dóra, Edina Brigitta Udvari, Dávid Keller, Árpád Dobolyi, András H. Lékó, Rashmi Kumari
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
medicine.medical_specialty
Steroid hormone receptor
Mothers
RNA-Seq
Biology
Catalysis
Article
Inorganic Chemistry
lcsh:Chemistry
rat dams
03 medical and health sciences
0302 clinical medicine
Internal medicine
Gene expression
medicine
Animals
Physical and Theoretical Chemistry
hypothalamus
Maternal Behavior
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Messenger RNA
Sequence Analysis
RNA
Gene Expression Profiling
Organic Chemistry
Postpartum Period
Antagonist
General Medicine
steroid receptor
Preoptic Area
Computer Science Applications
Rats
Preoptic area
Androgen receptor
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
Hypothalamus
Receptors
Androgen
gene expression
Female
postpartum mother
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 4
International Journal of Molecular Sciences, Vol 22, Iss 1517, p 1517 (2021)
ISSN: 1422-0067
Popis: (1) Background: Preoptic region of hypothalamus is responsible to control maternal behavior, which was hypothesized to be associated with gene expressional changes. (2) Methods: Transcriptome sequencing was first applied in the preoptic region of rat dams in comparison to a control group of mothers whose pups were taken away immediately after parturition and did not exhibit caring behavior 10 days later. (3) Results: Differentially expressed genes were found and validated by quantitative RT-PCR, among them NACHT and WD repeat domain containing 1 (Nwd1) is known to control androgen receptor (AR) protein levels. The distribution of Nwd1 mRNA and AR was similar in the preoptic area. Therefore, we focused on this steroid hormone receptor and found its reduced protein level in rat dams. To establish the function of AR in maternal behavior, its antagonist was administered intracerebroventricularly into mother rats and increased pup-directed behavior of the animals. (4) Conclusions: AR levels are suppressed in the preoptic area of mothers possibly mediated by altered Nwd1 expression in order to allow sustained high-level care for the pups. Thus, our study first implicated the AR in the control of maternal behaviors.
Databáze: OpenAIRE
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