Biomolecules Immobilization Using the Aryl Diazonium Electrografting
| Autor: | Christophe A. Marquette, B. C. Corgier, Sofiene Abdellaoui, Bastien Doumèche, Céline A. Mandon, Loïc J. Blum |
|---|---|
| Přispěvatelé: | Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École Supérieure Chimie Physique Électronique de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, AXO Science (Axo S) |
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Oligonucleotide Biomolecule Aryl education Nanotechnology 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] 3. Good health 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Electrochemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] 0210 nano-technology Biochip Biosensor Macromolecule Label free |
| Zdroj: | Electroanalysis Electroanalysis, Wiley-VCH Verlag, 2013, 25 (3), pp.671-684. ⟨10.1002/elan.201200334⟩ Electroanalysis, Wiley-VCH Verlag, 2013, 25 (3), pp.671-684 |
| ISSN: | 1040-0397 1521-4109 |
| DOI: | 10.1002/elan.201200334 |
| Popis: | International audience; The electrografting of aryl-diazonium was proven to be an efficient way to immobilize antibodies, oligonucleotides and enzymes onto conductive supports. Biomolecules chemically functionalized with aniline derivates or diazotated derivates could be used to build complex architectures acting as sensing layers for biosensors, biochips or others bioelectronic devices. Additionally, the use of SPR offers new opportunities to characterize the grafted surfaces as well as to develop label-free assays. Here, we give an overview of our group achievements in the field during the last 7 years. We highlight the applications of these functionalized surfaces in multiparametric sandwich assays, in the label free detection and imaging of macromolecular interactions by SPRi and in the immobilization of dehydrogenase via its cofactor. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |