Association of polymorphisms of the ?-defensin 1 gene with nematode and protozoan infection traits in goat
| Autor: | Ednaldo da Silva Filho, José Elivalto Guimarães Campelo, José Ribamar Felipe Marques, Flora Suzane Parente Maia, Caio Santos Silva, Rafaelle Casseb Guimarães, José Lindemberg Rocha Sarmento, Francisco Arimatéia Santos Alves |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Polimorfismo Gen?tico / gen?tica beta-Defensins Genotype Nematoda 030231 tropical medicine Immunology Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Feces 03 medical and health sciences chemistry.chemical_compound Exon 0302 clinical medicine Polymorphism (computer science) Molecular marker Protozoan infection parasitic diseases medicine Animals SNP Genetic Predisposition to Disease Protozoan Infections Animal Gene Infec??es por Protozo?rios Genetics Base Sequence Goats Infec??es por Nematoides Sequence Analysis DNA medicine.disease 030104 developmental biology Amino Acid Substitution chemistry Cabras / parasitologia Female Parasitology Brazil |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| Popis: | Federal University of Piau?. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil. Federal University of Piau?. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil. Federal University of Piau?. Department of Animal Production. College of Veterinary Medicine. Teresina, PI, Brazil. Embrapa Eastern Amazon. Brazilian Agricultural Research Corporation. Bel?m, PA, Brazil. Embrapa Eastern Amazon. Brazilian Agricultural Research Corporation. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Federal Rural University of Amazon. Health and Animal Production Institute. Bel?m, PA, Brazil. Federal Rural University of Amazon. Health and Animal Production Institute. Bel?m, PA, Brazil. This study's purpose was to identify polymorphisms (SNP) in the goat ?-defensin 1 gene and to associate these SNPs with traits related to nematodean and protozoan infections in Anglo-Nubian goats from semiarid region of Brazil. A total of 184 animals were used for DNA extraction, PCR and DNA automatic sequencing. The association analyses included the fixed effects of animal age, bloodline and genotype of the SNP marker in the statistical model. The means of genotypes were compared by the Fisher test (P < 0.05). Twelve polymorphism genotypes were found: two in intron 1, seven in exon 2 and three in 3' untranslated region. The SNPs of exon 2 were responsible for amino acid substitutions in six genetic codes, and the changes in the 25th and 33rd codes affected the protein function. The SNP 1937 was significantly associated with number of protozoan oocysts, whereas SNP 2001 was associated with degree of anaemia (Famacha?). Polymorphism 2046, in turn, showed a significant association with Famacha? degree and number of protozoan oocysts. SNP 2140 associated with maximum EPG of the animal. Results from this study suggest that the ?-defensin 1 gene can be used as a molecular marker for selection of goats regarding the susceptibility to endoparasites infections. |
| Databáze: | OpenAIRE |
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