DISCORDANT PULMONARY PROINFLAMMATORY CYTOKINE EXPRESSION DURING ACUTE HYPEROXIA IN THE NEWBORN RABBIT
| Autor: | Rita M. Ryan, Sofia A. Chaudhry, Anna Paxhia, Michael B. LoMonaco, Carl T. D'Angio |
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| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
Chemokine Pathology medicine.medical_specialty Pulmonary Fibrosis medicine.medical_treatment Clinical Biochemistry Cell Count Enzyme-Linked Immunosorbent Assay In situ hybridization Hyperoxia Lung injury Proinflammatory cytokine Andrology Macrophages Alveolar medicine Animals RNA Messenger Interleukin 8 Lung Molecular Biology Chemokine CCL2 In Situ Hybridization biology Interleukin-8 Interleukin Epithelial Cells Blotting Northern Oxygen Pulmonary Alveoli Disease Models Animal Cytokine Animals Newborn biology.protein Rabbits medicine.symptom Bronchoalveolar Lavage Fluid Interleukin-1 |
| Zdroj: | Experimental Lung Research. 25:443-465 |
| ISSN: | 1521-0499 0190-2148 |
| DOI: | 10.1080/019021499270187 |
| Popis: | Newborn animals are resistant to oxygen toxicity. To investigate this phenomenon, the proinflammatory cytokines interleukin (IL)-1 beta, IL-8, and monocyte chemoattractant protein-1 (MCP-1) were measured during newborn rabbit hyperoxic lung injury. Pups were exposed to > 95% O2 for 8-9 days, followed by 60% O2 until 36 days of age. Lung lavage fluid, RNA, and tissue sections were collected at 0, 2, 4, 6, 8, 10, 12, 14, 22, and 36 days. Acute inflammation occurred by 6-10 days of hyperoxia, and fibrosis by 22 days. Northern hybridization of lung homogenates from hyperoxia-exposed pups showed elevated MCP-1 and IL-8 mRNA expression at 6 and 10 days, respectively, compared to age-matched, air-exposed controls. Lavage fluid IL-8 protein also peaked at 10 days, and was strongly correlated to neutrophil numbers in lavage. In situ hybridization revealed elevated IL-1 beta mRNA in macrophages, alveolar epithelial and interstitial cells at 2-10 days, elevated MCP-1 mRNA in similar cell types at 4-8 days, and elevated IL-8 mRNA in these cells and neutrophils at 4-10 days. IL-1 beta and IL-8 expression peaked during peak inflammation, whereas peak MCP-1 expression preceded macrophage influx. Comparing newborn and adult animals' chemokine response may help explain their differences in hyperoxia susceptibility. |
| Databáze: | OpenAIRE |
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