A New Surface Plasmon Resonance Assay for In Vitro Screening of Mannose-Binding Lectin Inhibitors
| Autor: | Matteo Stravalaci, Marco Gobbi, Alessandro Palmioli, Anna Bernardi, Carlo Perego, Daiana De Blasio, Franca Orsini, Maria Grazia De Simoni, Giulio Goti |
|---|---|
| Přispěvatelé: | Stravalaci, M, De Blasio, D, Orsini, F, Perego, C, Palmioli, A, Goti, G, Bernardi, A, De Simoni, M, Gobbi, M |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Dendrimers Carbohydrates Mannose chemical and pharmacologic phenomena Mannose-Binding Lectin Biochemistry Analytical Chemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Humans immunoassay Surface plasmon resonance Mannan-binding lectin mannose binding lectin biology medicine.diagnostic_test glycodendrimer Lectin inhibitor screening Surface Plasmon Resonance bacterial infections and mycoses Immunity Innate In vitro High-Throughput Screening Assays Complement system 030104 developmental biology chemistry Reperfusion Injury Lectin pathway Immunoassay biology.protein Molecular Medicine 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Europe PubMed Central |
| ISSN: | 2472-5552 |
| DOI: | 10.1177/1087057116637563 |
| Popis: | Mannose-binding lectin (MBL) is a circulating protein that acts as a soluble pattern recognition molecule of the innate immunity. It binds to carbohydrate patterns on the surface of pathogens or of altered self-cells, with activation of the lectin pathway of the complement system. Recent evidence indicates that MBL contributes to the pathophysiology of ischemia-reperfusion injury and other conditions. Thus, MBL inhibitors offer promising therapeutic strategies, since they prevent the interaction of MBL with its target sugar arrays. We developed and characterized a novel assay based on surface plasmon resonance for in vitro screening of these compounds, which may be useful before the more expensive and time-consuming in vivo studies. The assay measures the inhibitor's ability to interfere with the binding of murine MBL-A or MBL-C, or of human recombinant MBL, to mannose residues immobilized on the sensor chip surface. We have applied the assay to measure the IC50 of synthetic glycodendrimers, two of them with neuroprotective properties in animal models of MBL-mediated injuries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|