Induction of mouse p55 interleukin-2 receptor gene expression by IL-2 and IL-4 and characterization of its transcription initiation sites
| Autor: | Jacques Thèze, Patricia Chastagner, Dragana Lj. Jankovic, P. Froussard |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic T cell Immunology Molecular Sequence Data Gene Expression Biology Regulatory Sequences Nucleic Acid Cell Line Mice Cell surface receptor Interleukin-4 receptor Gene expression medicine Animals RNA Messenger Receptor Molecular Biology Base Sequence GATA3 Receptors Interleukin-2 Molecular biology Recombinant Proteins Interleukin 10 medicine.anatomical_structure Genes Interleukin-21 receptor Interleukin-2 Interleukin-4 |
| Zdroj: | Molecular immunology. 28(1-2) |
| ISSN: | 0161-5890 |
| Popis: | Two murine T cell lines (C30.1 and Line 1) were used to study the expression of the p55 interleukin-2 receptor gene. C30.1 is an IL-2-dependent T cell line that can be stimulated for a short period of time by IL-4. Line 1 cells are propagated in IL-4 but they also proliferate in response to IL-2. In both cell lines stimulation by IL-2 leads to a strong induction of p55 IL-2 receptor mRNA while stimulation by IL-4 leads only to a very moderate increase in expression of this mRNA. The induction of p55 IL-2 receptor mRNA by IL-4 is comparable to that of β-actin mRNA. These data confirm that IL-2 upregulates p55 IL-2 receptor gene expression while IL-4, which also activates T cells, does not lead to specific induction of this gene. We have also determined the transcription initiation sites utilized by the p55 IL-2 receptor gene in C30.1 and Line 1 cells. Seven sites were identified, one of which predominates. Resting cells, or cells stimulated with IL-2 or IL-4, display the same pattern of transcription site utilization. |
| Databáze: | OpenAIRE |
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