A novel voltammetry offline coupled MALDI/TOF MS characterization of electrochemical reaction products and the voltammetric determination of febuxostat in human plasma
| Autor: | Essam F. Khamis, Fawzi A. El-Yazbi, Sameh E. Younis, Omayma A. Amin, Eman I. El-Kimary, Rania Bakry |
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| Rok vydání: | 2019 |
| Předmět: |
Xanthine Oxidase
Nitrile Carboxylic Acids 02 engineering and technology Electrochemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Febuxostat Nitriles medicine Humans Voltammetry Chromatography Plasma samples Chemistry 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Matrix-assisted laser desorption/ionization Human plasma Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Time-of-flight mass spectrometry 0210 nano-technology Blood Chemical Analysis medicine.drug |
| Zdroj: | Talanta. 194:542-547 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2018.10.087 |
| Popis: | A simple offline coupling voltammetry-MALDI/TOF MS procedure is presented for studying electrochemical reactions. It was utilized for the characterization of the electro-reduction products of febuxostat in methanolic acetate buffer (0.1 M, pH 5). The MS analysis reveals that the carboxylic and nitrile groups are the electro-reducible groups at −0.9338 and −1.5503 V with the conversion to aldehydic and amino groups, respectively. The developed voltammetric method was validated and applied successfully for the drug determination in pharmaceutical tablets and real plasma samples within the linearity ranges 0.03–2 and 0.4–5 μg mL−1, respectively. |
| Databáze: | OpenAIRE |
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