High-dimensional, single-cell characterization of the brain's immune compartment
| Autor: | Tania Dubovik, Tamar Koren, Maya Schiller, Hilla Azulay-Debby, Tamar L. Ben-Shaanan, Asya Rolls, Ben Korin, Nadia Boshnak |
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| Rok vydání: | 2017 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine animal diseases chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Biology Mice 03 medical and health sciences 0302 clinical medicine Immune system Animals Mass cytometry Cluster of differentiation General Neuroscience Lymphokine Brain Dendritic Cells biochemical phenomena metabolism and nutrition Flow Cytometry Acquired immune system Mice Inbred C57BL B-1 cell Hyaluronan Receptors 030104 developmental biology Neuroimmunology Immunology bacteria Choroid plexus 030217 neurology & neurosurgery |
| Zdroj: | Nature Neuroscience. 20:1300-1309 |
| ISSN: | 1546-1726 1097-6256 |
| DOI: | 10.1038/nn.4610 |
| Popis: | Korin et al. use CyTOF mass cytometry to characterize immune cell populations in the naive mouse brain (parenchyma, choroid plexus and meninges). This single-cell analysis of cell-surface proteins reveals the presence and phenotype of distinctive immune populations in the mouse brain compartment. The brain and its borders create a highly dynamic microenvironment populated with immune cells. Yet characterization of immune cells within the naive brain compartment remains limited. In this study, we used CyTOF mass cytometry to characterize the immune populations of the naive mouse brain using 44 cell surface markers. By comparing immune cell composition and cell profiles between the brain compartment and blood, we were able to characterize previously undescribed cell subsets of CD8 T cells, B cells, NK cells and dendritic cells in the naive brain. Using flow cytometry, we show differential distributions of immune populations between meninges, choroid plexus and parenchyma. We demonstrate the phenotypic ranges of resident myeloid cells and identify CD44 as a marker for infiltrating immune populations. This study provides an approach for a system-wide view of immune populations in the brain and is expected to serve as a resource for understanding brain immunity. |
| Databáze: | OpenAIRE |
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